Tang Li, Zhang Shuo, Zhang Min, Wang Pengjiao, Liang Guiyou, Gan Zhitong, Gao Xiuli
State Key Laboratory of Functions and Applications of Medicinal Plants, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang, China.
Microbiology and Biochemical Pharmaceutical Engineering Research Center of Guizhou Provincial Department of Education, Guizhou Medical University, Guiyang, China.
Front Microbiol. 2024 Jan 11;15:1351295. doi: 10.3389/fmicb.2024.1351295. eCollection 2024.
Acute lung injury (ALI) is a serious respiratory disease characterized by progressive respiratory failure with high morbidity and mortality. It is becoming increasingly important to develop functional foods from polyphenol-rich medicinal and dietary plants in order to prevent or alleviate ALI by regulating intestinal microflora. Rosa roxburghii Tratt polyphenol (RRTP) has significant preventive and therapeutic effects on lipopolysaccharide-induced ALI mice, but its regulatory effects on gut homeostasis in ALI mice remains unclear.
This study aims to systematically evaluate the ameliorative effects of RRTP from the perspective of "lung-gut axis" on ALI mice by intestine histopathological assessment, oxidative stress indicators detection and short-chain fatty acids (SCFAs) production, and then explore the modulatory mechanisms of RRTP on intestinal homeostasis by metabolomics and gut microbiomics of cecal contents.
The results showed that RRTP can synergistically exert anti-ALI efficacy by significantly ameliorating intestinal tissue damage, inhibiting oxidative stress, increasing SCFAs in cecal contents, regulating the composition and structure of intestinal flora, increasing Akkermansia muciniphila and modulating disordered intestinal endogenous metabolites.
This study demonstrated that RRTP has significant advantages in adjuvant therapy of ALI, and systematically clarified its comprehensive improvement mechanism from a new perspective of "lung-gut axis", which provides a breakthrough for the food and healthcare industries to develop products from botanical functional herbs and foods to prevent or alleviate ALI by regulating intestinal flora.
急性肺损伤（ALI）是一种严重的呼吸系统疾病，其特征为进行性呼吸衰竭，发病率和死亡率高。从富含多酚的药用和食用植物中开发功能性食品，以通过调节肠道微生物群来预防或减轻ALI变得越来越重要。刺梨多酚（RRTP）对脂多糖诱导的ALI小鼠具有显著的预防和治疗作用，但其对ALI小鼠肠道稳态的调节作用仍不清楚。
本研究旨在从“肺-肠轴”的角度，通过肠道组织病理学评估、氧化应激指标检测和短链脂肪酸（SCFAs）生成，系统评价RRTP对ALI小鼠的改善作用，然后通过盲肠内容物的代谢组学和肠道微生物组学探索RRTP对肠道稳态的调节机制。
结果表明，RRTP可通过显著改善肠道组织损伤、抑制氧化应激、增加盲肠内容物中的SCFAs、调节肠道菌群的组成和结构、增加嗜黏蛋白阿克曼氏菌以及调节紊乱的肠道内源性代谢产物，协同发挥抗ALI功效。
本研究表明RRTP在ALI的辅助治疗中具有显著优势，并从“肺-肠轴”的新角度系统阐明了其综合改善机制，为食品和保健行业从植物功能性草药和食品开发产品以通过调节肠道菌群预防或减轻ALI提供了突破。
