Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
Chem Commun (Camb). 2024 Feb 13;60(14):1948-1951. doi: 10.1039/d3cc06192b.
Nucleic acids in blood are early indicators of disease that could be detected by point-of-care biosensors if sufficiently sensitive and facile sensors existed. Electrochemical hybridization assays are sensitive and specific but are limited to very short nucleic acids. We have developed a restriction enzyme-assisted electrochemical hybridization (REH) assay for improved nucleic acid detection. By incorporating target-specific restriction enzymes, we detect long nucleic acids, with performance dependent on the location of the cut site relative to the electrode surface. Thus, we have further established guidelines for REH design to serve as a generalizable platform for robust electrochemical detection of long nucleic acids.
血液中的核酸是疾病的早期指标，如果存在足够敏感和简便的生物传感器，就可以通过即时检测生物传感器进行检测。电化学杂交分析具有灵敏度高、特异性强的特点，但只能检测非常短的核酸。我们开发了一种基于限制酶的电化学杂交（REH）检测方法，以提高核酸检测的灵敏度。通过引入靶标特异性限制酶，我们可以检测到长核酸，其性能取决于切割位点相对于电极表面的位置。因此，我们进一步制定了 REH 设计的指导方针，作为一种通用的电化学检测长核酸的平台。
