School of Biological Sciences, Department of Cell and Developmental Biology, University of California, Berkeley, Berkeley, United States.
Division of Biology and Biological Engineering (BBE), California Institute of Technology, Pasadena, United States.
Elife. 2024 Jan 30;12:RP90199. doi: 10.7554/eLife.90199.
Each year, hundreds of millions of people are infected with arboviruses such as dengue, yellow fever, chikungunya, and Zika, which are all primarily spread by the notorious mosquito . Traditional control measures have proven insufficient, necessitating innovations. In response, here we generate a next-generation CRISPR-based precision-guided sterile insect technique (pgSIT) for that disrupts genes essential for sex determination and fertility, producing predominantly sterile males that can be deployed at any life stage. Using mathematical models and empirical testing, we demonstrate that released pgSIT males can effectively compete with, suppress, and eliminate caged mosquito populations. This versatile species-specific platform has the potential for field deployment to effectively control wild populations of disease vectors.
每年，数亿人感染黄病毒，如登革热、黄热病、基孔肯雅热和寨卡病毒，这些病毒主要由臭名昭著的蚊子传播。传统的控制措施已经证明是不够的，需要创新。有鉴于此，我们在这里开发了一种基于下一代 CRISPR 的精确制导不育昆虫技术 (pgSIT)，该技术破坏性别决定和生育所必需的基因，产生主要是不育的雄性，可以在任何生命阶段释放。通过数学模型和实证测试，我们证明释放的 pgSIT 雄性可以有效地与笼养蚊子种群竞争、抑制和消灭。这种多功能的种特异性平台具有现场部署的潜力，可以有效地控制疾病媒介的野生种群。
