Moghan Mavheena, Naidu Paarvaishnee, Zulkifly Nadhrah Binti, Selan Yulfia Nelymalik, Setyawan Erif Maha Nugraha, Winarsih Sugi, Kusindarta Dwi Liliek
Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Department of Anatomy, Faculty of Medicine and Veterinary Medicine, Nusa Cendana University, Kupang, Indonesia.
Open Vet J. 2023 Dec;13(12):1597-1606. doi: 10.5455/OVJ.2023.v13.i12.9. Epub 2023 Dec 31.
Burn injuries are an alarming indicator of the sensitivity of human tissue when confronted with high temperatures or chemicals. The current treatment for burn wounds needs to be improved and more extensive in scope. Significant research advances concerning the therapeutic potential of secretomes over the past two decades have expanded the range of therapies that utilize secretomes to encompass populations other than stem cells.
This study details how the secretome extracted from the bovine umbilical vein endothelial cell (BUVEC) promotes the healing of burn injuries.
The 48 rats were divided into four groups, namely the control group with povidone-iodine, the 5% BUVEC-conditioned medium (CM) cream group, the 10% BUVEC-CM cream group, the 15% BUVEC-CM cream group. Animals induced type II burns under anesthesia. Treatment is carried out topically, two times a day. Every day the wound was measured. The animals were put to sleep for samples on days 5, 13, 21, and 19. Samples in the form of skins were soaked in 4% paraformaldehyde and processed with paraffin-embedded for tissue preparations. The research results were processed using two way ANOVA.
The study showed that on day 5, wound closure occurred, whereas in the povidone-iodine group, macroscopically, the wound closed faster. Epithelial repair, increased fibroblasts and collagen, and blood vessel formation greatly increased in the 15% BUVEC-CM group on days 13, 21, and 29.
Taken together, BUVEC secretome promoted fibroblast regeneration, collagen formation, re-epithelialization, and hair follicle regeneration on the burn injury wound healing.
烧伤是人体组织在面对高温或化学物质时敏感性的一个警示指标。目前烧伤创面的治疗方法有待改进且范围需更广泛。在过去二十年中，关于分泌组治疗潜力的重大研究进展扩大了利用分泌组的治疗范围，使其涵盖除干细胞以外的其他群体。
本研究详细阐述了从牛脐静脉内皮细胞（BUVEC）中提取的分泌组如何促进烧伤创面的愈合。
将48只大鼠分为四组，即聚维酮碘对照组、5%BUVEC条件培养基（CM）乳膏组、10%BUVEC-CM乳膏组和15%BUVEC-CM乳膏组。动物在麻醉下诱导II度烧伤。进行局部治疗，每天两次。每天测量创面。在第5、13、21和19天处死动物取样本。将皮肤样本浸泡在4%多聚甲醛中，用石蜡包埋进行组织制备。研究结果采用双向方差分析进行处理。
研究表明，在第5天创面开始愈合，而在聚维酮碘组，从宏观上看创面愈合更快。在第13、21和29天，15%BUVEC-CM组的上皮修复、成纤维细胞和胶原蛋白增加以及血管形成显著增加。
综上所述，BUVEC分泌组在烧伤创面愈合过程中促进了成纤维细胞再生、胶原蛋白形成、再上皮化和毛囊再生。
