Laksmitawati Dian Ratih, Noor Siti Umrah, Sumiyati Yati, Hartanto Adrian, Widowati Wahyu, Pratami Diah Kartika
Laboratory of Biochemistry, Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia.
Laboratory of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Pancasila, Jakarta, 12640, Indonesia.
Vet World. 2022 Apr;15(4):841-847. doi: 10.14202/vetworld.2022.841-847. Epub 2022 Apr 7.
Stem cells are cells that can proliferate to form a new tissue, leading to its use in regenerative therapy. Stem cells will secrete biological factors, such as growth factors, cytokines, and other proteins to their surroundings and culture medium/conditioned medium (CM), altering tissue physiology. These factors can help wound healing, but their effect on third-degree burns is poorly understood. This research aimed to study the activity of mesenchymal stem cell-conditioned medium gel in healing and repairing third-degree burns on rats skin.
Twenty-four Sprague-Dawley rats with burn wounds on the dorsal area were divided into four groups; the first group was treated with CM gel, with a concentration equivalent to 0.05% protein, the second group was treated with a placebo gel, the third group with silver sulfadiazine (SSD) cream (SSD-Burnazin contain 10 mg/g SSD), and the fourth group was not given any treatment, for 21 days, and on the final day, the rats were sacrificed, and the skins were taken. All topical treatments completely cover the wound area.
Wound healing process indicators observed include wound diameter, scabs' formation, blister formation, and hair growth every day. The skins taken were processed with hematoxylin-eosin and Masson's trichrome staining. The indicators studied include neutrophil infiltration, mononuclear cell infiltration, neovascularization, collagen area, and re-epithelization ratio.
CM shows better wound healing than other groups and faster hair growth.
干细胞是能够增殖形成新组织的细胞，这使其可用于再生治疗。干细胞会向其周围环境以及培养基/条件培养基（CM）分泌生物因子，如生长因子、细胞因子和其他蛋白质，从而改变组织生理学。这些因子有助于伤口愈合，但其对三度烧伤的影响尚不清楚。本研究旨在探讨间充质干细胞条件培养基凝胶对大鼠三度烧伤皮肤的愈合和修复活性。
将24只背部有烧伤创面的Sprague-Dawley大鼠分为四组；第一组用浓度相当于0.05%蛋白质的CM凝胶治疗，第二组用安慰剂凝胶治疗，第三组用磺胺嘧啶银（SSD）乳膏（SSD-Burnazin含10mg/g SSD）治疗，第四组不进行任何治疗，持续21天，在最后一天，处死大鼠并取下皮肤。所有局部治疗均完全覆盖伤口区域。
观察的伤口愈合过程指标包括每天的伤口直径、结痂形成、水泡形成和毛发生长情况。取下的皮肤用苏木精-伊红和Masson三色染色法进行处理。研究的指标包括中性粒细胞浸润、单核细胞浸润、新生血管形成、胶原面积和再上皮化率。
CM显示出比其他组更好的伤口愈合效果以及更快的毛发生长速度。
