Piao Yinglan, Li Shizhen, Chen Yiduo, Zhao Sisi, Piao Zhongyun, Wang Haiping
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Hortic Res. 2023 Dec 13;11(1):uhad261. doi: 10.1093/hr/uhad261. eCollection 2024 Jan.
Clubroot disease caused by () severely threatens the cultivation of Cruciferous plants, especially Chinese cabbage. Recently, resistance genes in plants have been reported to encode for a Ca-permeable channel in the plasma membrane, which can mediate the cytosolic Ca increase in plant cells upon pathogen attack. However, the downstream Ca sensor and decoder are still unknown. In this study, we identified the virulent and avirulent isolates (Pbs) of two near isogenic lines, CR 3-2 and CS 3-2, with CR 3-2 harboring clubroot resistant gene . The transcriptomic analysis was then conducted with CR 3-2 after inoculating with virulent isolate PbE and avirulent isolate Pb4. From the differentially expressed genes of transcriptomic data, we identified a Ca-sensor encoding gene, ., that was highly induced in CR 3-2 during infection by Pb4 but not by PbE. Moreover, GUS histochemical staining and subcellular localization analysis revealed that . was specifically expressed in the root hair cells of and encoded a putative Ca sensor localized in the plasma membrane. We also developed an assay to investigate the BraCRa-mediated hypersensitive response (HR) in tobacco leaves. The results suggest that BraCBL1.2 is involved in the BraCRa-mediated plant ETI immune response against . In addition, we verified that overexpression of enhanced clubroot resistance in . Collectively, our data identified the involvement of a Ca sensor in BraCRa-mediated clubroot resistance in Chinese cabbage, providing a theoretical basis for further research on the resistance of Chinese cabbage to .
由（）引起的根肿病严重威胁十字花科植物的种植，尤其是大白菜。最近有报道称，植物中的抗性基因编码质膜中的一种钙通透通道，该通道可在病原体攻击时介导植物细胞胞质钙的增加。然而，下游的钙传感器和解码器仍然未知。在本研究中，我们鉴定了两个近等基因系CR 3-2和CS 3-2的致病和无毒分离株（Pbs），其中CR 3-2携带根肿病抗性基因。然后用致病分离株PbE和无毒分离株Pb4接种CR 3-2后进行转录组分析。从转录组数据的差异表达基因中，我们鉴定了一个钙传感器编码基因，即，该基因在Pb4感染期间在CR 3-2中高度诱导，但在PbE感染时未被诱导。此外，GUS组织化学染色和亚细胞定位分析表明，在的根毛细胞中特异性表达，并编码一种推定的位于质膜的钙传感器。我们还开发了一种检测方法来研究烟草叶片中BraCRa介导的过敏反应（HR）。结果表明，BraCBL1.2参与了BraCRa介导的植物对的ETI免疫反应。此外，我们验证了的过表达增强了对根肿病的抗性。总的来说，我们的数据确定了一种钙传感器参与了BraCRa介导的大白菜对根肿病的抗性，为进一步研究大白菜对的抗性提供了理论基础。
