Al-Khdour Muntaha S, Khabour Omar F, Al-Eitan Laith N, Alzoubi Karem H
Department of Applied Biological Sciences, Faculty of Science, Jordan University of Science and Technology, Irbid, Jordan.
Department of Medical Laboratory Sciences, Faculty of Medical Sciences, Jordan University of Science and Technology, Irbid, Jordan.
Drug Chem Toxicol. 2023 Jan;46(1):176-180. doi: 10.1080/01480545.2021.2015369. Epub 2021 Dec 29.
Nedaplatin is a chemotherapeutic agent used widely in cancer therapy. Nedaplatin has been shown to cause DNA damage to cells via the induction of oxidative stress. Vitamin E (Vit E) has an anti-mutagenic activity that can protect cells from DNA damaging agents. The objective of this study is to examine the genotoxic and cytotoxic effects of nedaplatin in human cultured lymphocytes. In addition, modulation of such effects by Vit E was also examined. The frequencies of sister chromatid exchange (SCE) and chromosomal aberrations (CAs) were used as an indicator for genotoxicity. The mitotic and proliferative indices were used to examine the cytotoxic effects of nedaplatin. The results showed that nedaplatin significantly elevated SCE and CA frequencies in human lymphocytes ( ˂ 0.01). The increases in the frequencies of SCE and CA caused by nedaplatin were lowered by pretreatment treatment with Vit E ( < 0.05). Nedaplatin significantly lowered mitotic index but Vit E pretreatment did not modulate this effect. These results suggest that Vit E has the potential to ameliorate the genotoxicity of nedaplatin in cultured lymphocytes.
奈达铂是一种广泛应用于癌症治疗的化疗药物。已表明奈达铂可通过诱导氧化应激对细胞造成DNA损伤。维生素E（Vit E）具有抗诱变活性，可保护细胞免受DNA损伤剂的影响。本研究的目的是检测奈达铂对人培养淋巴细胞的遗传毒性和细胞毒性作用。此外，还研究了Vit E对这些作用的调节。姐妹染色单体交换（SCE）频率和染色体畸变（CA）频率用作遗传毒性指标。有丝分裂指数和增殖指数用于检测奈达铂的细胞毒性作用。结果表明，奈达铂显著提高了人淋巴细胞中的SCE和CA频率（P＜0.01）。Vit E预处理降低了奈达铂引起的SCE和CA频率的增加（P＜0.05）。奈达铂显著降低了有丝分裂指数，但Vit E预处理并未调节此作用。这些结果表明，Vit E有潜力改善奈达铂在培养淋巴细胞中的遗传毒性。
