Jacobson Kathryn R, Saleh Aya M, Lipp Sarah N, Tian Chengzhe, Watson Audrey R, Luetkemeyer Callan M, Ocken Alexander R, Spencer Sabrina L, Kinzer-Ursem Tamara L, Calve Sarah
Purdue University Interdisciplinary Life Science Program, Purdue University, West Lafayette, IN 47907, USA.
Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA.
iScience. 2024 Jan 9;27(2):108838. doi: 10.1016/j.isci.2024.108838. eCollection 2024 Feb 16.
The extracellular matrix (ECM) is an integral part of multicellular organisms, connecting different cell layers and tissue types. During morphogenesis and growth, tissues undergo substantial reorganization. While it is intuitive that the ECM remodels in concert, little is known regarding how matrix composition and organization change during development. Here, we quantified ECM protein dynamics in the murine forelimb during appendicular musculoskeletal morphogenesis (embryonic days 11.5-14.5) using tissue fractionation, bioorthogonal non-canonical amino acid tagging, and mass spectrometry. Our analyses indicated that ECM protein (matrisome) composition in the embryonic forelimb changed as a function of development and growth, was distinct from other developing organs (brain), and was altered in a model of disease (). Additionally, the tissue distribution for select matrisome was assessed via immunohistochemistry in the wild-type embryonic and postnatal musculoskeletal system. This resource will guide future research investigating the role of the matrisome during complex tissue development.
细胞外基质（ECM）是多细胞生物不可或缺的一部分，连接着不同的细胞层和组织类型。在形态发生和生长过程中，组织会经历重大的重组。虽然直观上认为ECM会协同重塑，但对于发育过程中基质组成和组织如何变化却知之甚少。在这里，我们使用组织分级分离、生物正交非经典氨基酸标记和质谱法，对小鼠前肢在附肢肌肉骨骼形态发生过程中（胚胎第11.5 - 14.5天）的ECM蛋白动态进行了量化。我们的分析表明，胚胎前肢中的ECM蛋白（基质体）组成随发育和生长而变化，与其他发育中的器官（脑）不同，并且在疾病模型中发生了改变。此外，通过免疫组织化学在野生型胚胎和出生后肌肉骨骼系统中评估了选定基质体的组织分布。这一资源将指导未来研究基质体在复杂组织发育过程中的作用。
