Zhang Yu, Wang Lu, Ma Ning, Wan Yizhen, Zhu Xueyi, Qian Weiping
State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Langmuir. 2023 Aug 15;39(32):11406-11413. doi: 10.1021/acs.langmuir.3c01266. Epub 2023 Aug 5.
Nonspecific adsorption (NSA) seems to be an impregnable obstacle to the progress of the biomedical, diagnostic, microelectronic, and material fields. The reaction path of bioconjugation can alter the surface charge distribution on products and the interaction of bioconjugates, an ignored factor causing NSA. We monitored exacerbated NSA introduced by a 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide (EDC) addition reaction, which cannot be resistant to bovine serum albumin (BSA) or polyethylene glycol (PEG) antifouling coating and Tween-20. And the negative effects can be minimized by adding as low as 7.5 × 10 M -hydroxysulfosuccinimide (sulfo-NHS). We applied ordered porous layer interferometry (OPLI) to sensitively evaluate the NSA that is difficult to measure on individual particles. Using the silica colloidal crystal (SCC) film with Fabry-Perot fringes as in situ and real-time monitoring for the NSA, we optimized the surface chemistry to yield a conjugate surface without variational charge distribution. In this work, we propose a novel approach from the perspective of the reaction pathway to minimize the NSA of solely EDC-induced chemistry.
非特异性吸附（NSA）似乎是生物医学、诊断、微电子和材料领域发展中难以逾越的障碍。生物共轭反应路径会改变产物表面电荷分布以及生物共轭物之间的相互作用，这是导致NSA的一个被忽视的因素。我们监测了因添加1-乙基-3-（3-（二甲基氨基）丙基）碳二亚胺（EDC）反应而加剧的NSA，这种反应无法抵抗牛血清白蛋白（BSA）或聚乙二醇（PEG）防污涂层以及吐温-20。并且，通过添加低至7.5×10⁻³ M的羟基磺基琥珀酰亚胺（磺基-NHS），负面影响可以降至最低。我们应用有序多孔层干涉术（OPLI）来灵敏地评估单个颗粒上难以测量的NSA。使用具有法布里-珀罗条纹的二氧化硅胶体晶体（SCC）膜对NSA进行原位实时监测，我们优化了表面化学性质，以获得电荷分布无变化的共轭表面。在这项工作中，我们从反应路径的角度提出了一种新颖的方法，以最小化仅由EDC诱导的化学反应产生的NSA。
