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利用光干涉法实时监测负载在硅胶胶体晶体薄膜中的植物油的水解过程。

Real-time monitoring of the hydrolysis of vegetable oils loaded in silica colloidal crystal films with lipase by optical interferometry.

机构信息

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

出版信息

Anal Methods. 2022 Aug 18;14(32):3071-3078. doi: 10.1039/d2ay00811d.

DOI:10.1039/d2ay00811d
PMID:35924567
Abstract

Different vegetable oils have different nutritional components, especially in terms of the composition of their fatty acids, which can only be reflected after entering the human body. Therefore, when judging their health value and identifying high-quality vegetable oils, in addition to the analysis of their ingredients, tracking their hydrolysis process in the human body is a very important aspect. However, most identification methods or simulated digestion studies fail to achieve this. In this paper, we applied ordered porous layer interferometry (OPLI) for the real-time monitoring of optical thickness changes (ΔOT) to track the hydrolysis process of four kinds of vegetable oil. Further, this study can obtain precise data (the initial rate and degree of hydrolysis) to provide more information on the hydrolysis ability of different vegetable oils and give references for their nutritional and functional evaluation. In addition, it provides more possibilities for the adulteration identification and bioavailability analysis of vegetable oils.

摘要

不同的植物油具有不同的营养成分,特别是在脂肪酸的组成方面,这些成分只有在进入人体后才能体现出来。因此,在判断它们的健康价值和识别优质植物油时,除了对其成分进行分析外,跟踪它们在人体中的水解过程也是一个非常重要的方面。然而,大多数鉴定方法或模拟消化研究都无法做到这一点。在本文中,我们应用有序多孔层干涉仪(OPLI)实时监测光学厚度变化(ΔOT),以跟踪四种植物油的水解过程。此外,本研究可以获得精确的数据(初始水解速率和水解程度),为不同植物油的水解能力提供更多信息,并为其营养和功能评价提供参考。此外,它为植物油的掺假鉴别和生物利用度分析提供了更多的可能性。

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引用本文的文献

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From Self-Assembly of Colloidal Crystals toward Ordered Porous Layer Interferometry.从胶体晶体的自组装到有序多孔层干涉测量。
Biosensors (Basel). 2023 Jul 13;13(7):730. doi: 10.3390/bios13070730.