Ma Ning, Wan Yizhen, Zhou Lele, Wang Lu, Qian Weiping
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Int J Biol Macromol. 2022 Apr 1;203:563-571. doi: 10.1016/j.ijbiomac.2022.01.185. Epub 2022 Feb 1.
Polysaccharides and proteins have attracted increasing interest in the fields of biomedicine and green chemical as biocomposites due to their inherent versatility. Here, we used silica colloidal crystal (SCC) films combined with an ordered porous layer interferometry (OPLI) method to investigate the interaction between chitosan and pepsin at different concentrations and pH values in real time. Zeta potential was combined with attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and Fourier transform infrared microscopy (FTIR microscopy) to illustrate the interaction mechanism further. The results showed that the variation and slope of the optical thickness (OT) caused by the Fabry-Perot fringes represent the degree and process of interaction. The protonation of chitosan and the net charge carried by pepsin caused various degrees of electrostatic attraction under different pH values. Meanwhile, the rate and degree of hydrolysis were positively correlated with pepsin concentration. This work results provide a theoretical basis for designing novel composites based on the development of polysaccharides and proteins.
由于多糖和蛋白质具有固有的多功能性，它们作为生物复合材料在生物医学和绿色化学领域引起了越来越多的关注。在此，我们使用二氧化硅胶体晶体（SCC）薄膜结合有序多孔层干涉测量法（OPLI）实时研究壳聚糖和胃蛋白酶在不同浓度和pH值下的相互作用。将zeta电位与衰减全反射傅里叶变换红外光谱（ATR-FTIR）和傅里叶变换红外显微镜（FTIR显微镜）相结合，以进一步阐明相互作用机制。结果表明，由法布里-珀罗条纹引起的光学厚度（OT）的变化和斜率代表了相互作用的程度和过程。壳聚糖的质子化以及胃蛋白酶携带的净电荷在不同pH值下引起了不同程度的静电吸引。同时，水解速率和程度与胃蛋白酶浓度呈正相关。这项工作的结果为基于多糖和蛋白质开发设计新型复合材料提供了理论依据。
