Department of Anesthesiology and Intensive care, Faculty of Medicine, Tabriz University of Medical Science, Tabriz, Iran.
Department of Biological Sciences, Faculty of Basic Sciences, Higher Education Institute of Rab- Rashid, Tabriz, Iran.
Mol Biol Rep. 2024 Feb 7;51(1):282. doi: 10.1007/s11033-024-09225-x.
Sepsis is a life-threatening condition where early diagnosis and prognostic awareness provide guidance for selecting the appropriate treatment strategies. A wide variety of biomarker-based studies in clinical medicine provide new insights into personalized medicine for sepsis patients. MiRNAs are endogenous non-coding RNA molecules that have been acting as great potential diagnostic, prognostic and therapeutic biomarkers in various diseases.
In the present study, the expression levels of two selected miRNAs, including miR-135a and miR-193, were evaluated for their prognostic potential in patients with sepsis. The circulating levels of miRNAs were quantified by quantitative PCR (qPCR) in patients with sepsis (n = 100) and age- and sex-matched healthy controls (n = 100). Statistical findings confirmed the valuable prognostic potential of miR-135a in patients with sepsis, while no significant difference was found between the miR-193 expression level in the patients with sepsis and the controls.
Circulating levels of miRNA-135a can serve a the prognostic biomarker for patients with sepsis. These findings highlight the importance of miRNAs as signatures in the personalized managements of sepsis.
脓毒症是一种危及生命的病症，早期诊断和预后意识为选择合适的治疗策略提供了指导。临床中大量基于生物标志物的研究为脓毒症患者的个性化医学提供了新的见解。miRNA 是内源性非编码 RNA 分子，在各种疾病中作为极具潜力的诊断、预后和治疗生物标志物发挥作用。
本研究评估了两种选定的 miRNA（miR-135a 和 miR-193）在脓毒症患者中的预后潜力。采用定量 PCR（qPCR）方法定量检测脓毒症患者（n=100）和年龄、性别匹配的健康对照者（n=100）的 miRNA 循环水平。统计结果证实 miR-135a 在脓毒症患者中有重要的预后价值，而脓毒症患者与对照组 miR-193 的表达水平无显著差异。
循环 miRNA-135a 水平可作为脓毒症患者的预后生物标志物。这些发现强调了 miRNA 作为脓毒症个体化管理标志物的重要性。
