Anish Rajamohanan Jalaja, Nair Aswathy, Saraswathy V, Kalpana Velappan Nair S, Shyma Rajendran L
Department of Biochemistry, University of Kerala, Kariyavattom Campus, Thiruvananthapuram, Kerala, 695581, India.
Kerala State Palmyrah Products Development and Workers' Welfare Corporation Limited, Trivandrum, India.
Lab Anim Res. 2024 Feb 7;40(1):2. doi: 10.1186/s42826-024-00191-w.
Pterospermum rubiginosum has been traditionally used by the tribal inhabitants of Southern India for treating bone fractures and as a local anti-inflammatory agent; however, experimental evidence to support this traditional usage is lacking. The present study aimed to investigate the phytochemical characterization, in silico and in vitro anti-inflammatory evaluation, followed by in vivo toxicological screening of P. rubiginosum methanolic bark extract (PRME).
The LCMS evaluation revealed the presence of 80 significant peaks; nearly 50 molecules were identified using the LCMS database. In silico analysis showed notable interactions with inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6). In vitro gene expression study supported the docking results with significant down-regulation of iNOS, IL-6, and IL-10. PRME was administered orally to the SD rats and was found to be non-toxic up to 1000 mg/kg body weight for 14 days. The antioxidant enzymes catalase and sodium dismutase exhibited an increased value in PRME-administered groups, possibly due to the diverse phytochemical combinations in bark extract.
PRME administration significantly downregulated the gene expression of inflammatory markers, such as iNOS, IL-6, and IL-10. The molecular docking analysis of iNOS and IL-6 supports the in vitro study. In vivo toxicological study of PRME in SD rats was found to be non-toxic up to a concentration of 1000 mg/kg body weight for 14 days.
印度南部的部落居民传统上使用锈毛翅子树来治疗骨折，并将其作为局部抗炎剂；然而，缺乏支持这种传统用法的实验证据。本研究旨在调查锈毛翅子树甲醇树皮提取物（PRME）的植物化学特征、计算机模拟和体外抗炎评估，随后进行体内毒理学筛查。
液相色谱-质谱联用（LCMS）评估显示存在80个显著峰；使用LCMS数据库鉴定了近50种分子。计算机模拟分析表明与诱导型一氧化氮合酶（iNOS）和白细胞介素-6（IL-6）有显著相互作用。体外基因表达研究支持对接结果，iNOS、IL-6和IL-10显著下调。将PRME口服给予SD大鼠，发现其在14天内高达1000mg/kg体重时无毒。抗氧化酶过氧化氢酶和超氧化物歧化酶在给予PRME的组中值增加，可能是由于树皮提取物中多种植物化学组合。
给予PRME可显著下调iNOS、IL-6和IL-10等炎症标志物的基因表达。iNOS和IL-6的分子对接分析支持体外研究。对SD大鼠进行的PRME体内毒理学研究发现，在14天内浓度高达1000mg/kg体重时无毒。
