Development and Collaborative Innovation Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.
Sci Rep. 2024 Feb 8;14(1):3233. doi: 10.1038/s41598-024-53798-3.
The root system is important for the growth and development of spinach. To reveal the temporal variability of the spinach root system, root traits of 40 spinach accessions were measured at three imaging times (20, 30, and 43 days after transplanting) in this study using a non-destructive and non-invasive root analysis system. Results showed that five root traits were reliably measured by this system (RootViz FS), and two of which were highly correlated with manually measured traits. Root traits had higher variations than shoot traits among spinach accessions, and the trait of mean growth rate of total root length had the largest coefficients of variation across the three imaging times. During the early stage, only tap root length was weakly correlated with shoot traits (plant height, leaf width, and object area (equivalent to plant surface area)), whereas in the third imaging, root fresh weight, total root length, and root area were strongly correlated with shoot biomass-related traits. Five root traits (total root length, tap root length, total root area, root tissue density, and maximal root width) showed high variations with coefficients of variation values (CV ≥ 0.3, except maximal root width) and high heritability (H > 0.6) among the three stages. The 40 spinach accessions were classified into five subgroups with different growth dynamics of the primary and lateral roots by cluster analysis. Our results demonstrated the potential of in-situ phenotyping to assess dynamic root growth in spinach and provide new perspectives for biomass breeding based on root system ideotypes.
根系对菠菜的生长和发育很重要。为了揭示菠菜根系的时间变化性，本研究采用一种无损、非侵入性的根系分析系统，在移栽后 20、30 和 43 天三个成像时间点测量了 40 份菠菜种质的根系特性。结果表明，该系统（RootViz FS）可可靠测量 5 个根系特性，其中 2 个与手动测量特性高度相关。根系特性在菠菜种质间的变异性高于地上部特性，总根长平均生长率的性状在三个成像时间点的变异系数最大。在早期，只有主根长与地上部特性（株高、叶宽和物体面积（相当于植物表面积））呈弱相关，而在第三个成像时，根鲜重、总根长和根面积与地上部生物量相关特性呈强相关。5 个根系特性（总根长、主根长、总根面积、根组织密度和最大根宽）在三个阶段均表现出较高的变异性（变异系数值 CV≥0.3，最大根宽除外）和较高的遗传力（H＞0.6）。通过聚类分析，将 40 份菠菜种质分为主根和侧根生长动态不同的 5 个亚组。本研究结果表明，原位表型分析在评估菠菜动态根系生长方面具有潜力，并为基于根系理想型的生物量育种提供了新的视角。
