Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
Scar Laser and Plastic Surgery Center, Yonsei Cancer Hospital, Seoul 03722, Republic of Korea.
Int J Mol Sci. 2024 Feb 4;25(3):1885. doi: 10.3390/ijms25031885.
Hair luster is a key attribute of healthy hair and a crucial aspect of cosmetic appeal, reflecting the overall health and vitality of hair. Despite its significance, the advancement of therapeutic strategies for hair luster enhancement have been limited due to the absence of an effective experimental model. This study aimed to establish a novel animal model to assess hair gloss, employing ultraviolet (UV) irradiation on C57BL/6 mice. Specifically, UVB irradiation was meticulously applied to the shaved skin of these mice, simulating conditions that typically lead to hair luster loss in humans. The regrowth and characteristics of the hair were evaluated using a dual approach: an Investigator's Global Assessment (IGA) scale for subjective assessment and an image-based pixel-count method for objective quantification. These methods provided a comprehensive understanding of the changes in hair quality post-irradiation. To explore the potential reversibility of hair luster changes, oral minoxidil was administered, a treatment known for its effects on hair growth and texture. Further, to gain insights into the underlying biological mechanisms, bulk RNA transcriptomic analysis of skin tissue was conducted. This analysis revealed significant alterations in the expression of keratin-associated protein (KRTAP) genes, suggesting modifications in hair keratin crosslinking due to UV exposure. These changes are crucial in understanding the molecular dynamics affecting hair luster. The development of this new mouse model is a significant advancement in hair care research. It not only facilitates the evaluation of hair luster in a controlled setting but also opens avenues for the research and development of innovative therapeutic strategies. This model holds promise for the formulation of more effective hair care products and treatments, potentially revolutionizing the approach towards managing and enhancing hair luster.
头发光泽是健康头发的关键属性，也是美容吸引力的重要方面，反映了头发的整体健康和活力。尽管其意义重大，但由于缺乏有效的实验模型，头发光泽增强的治疗策略的进展一直受到限制。本研究旨在建立一种新的动物模型来评估头发光泽，采用紫外线（UV）照射 C57BL/6 小鼠。具体来说，对这些小鼠的剃光皮肤进行精心的 UVB 照射，模拟通常导致人类头发光泽丧失的条件。使用主观评估的研究者全球评估（IGA）量表和基于图像的像素计数方法对头发的再生和特征进行客观量化。这些方法提供了对辐照后头发质量变化的全面了解。为了探索头发光泽变化的潜在可逆性，给予口服米诺地尔，这是一种已知对头发生长和质地有影响的治疗方法。此外，为了深入了解潜在的生物学机制，对皮肤组织进行了大量 RNA 转录组分析。该分析显示角蛋白相关蛋白（KRTAP）基因的表达发生了显著改变，表明由于 UV 暴露导致头发角蛋白交联发生变化。这些变化对于理解影响头发光泽的分子动态至关重要。这种新小鼠模型的开发是头发护理研究的重大进展。它不仅在受控环境中便于评估头发光泽，而且为研究和开发创新治疗策略开辟了途径。该模型有望为更有效的头发护理产品和治疗方法的制定提供可能，从而彻底改变管理和增强头发光泽的方法。
