State Scientific Center of the Russian Federation - Institute for Biomedical Problems, Russian Academy of Sciences, Moscow, Russia.
Bull Exp Biol Med. 2024 Jan;176(3):394-398. doi: 10.1007/s10517-024-06031-0. Epub 2024 Feb 12.
In order to identify changes in the blood proteome of healthy volunteers after passive tilt test carried out on day 19 of head-down bed rest, a chromato-mass-spectrometric analysis of samples of dried blood spots was carried out. It was revealed that the body's response to the tilt test was characterized by a decrease in the level of HDL and kininogen-1. After the tilt test, we observed an increase in the level of vimentin, vitamin K-dependent protein C, Wnt signaling pathway proteins, proteins involved in autophagy and adaptive immune response, focal adhesion proteins, vascular damage marker S100A8, PEDF regulator, and some proteins of the heart: cardiac actin ACTC1 and transcription factor GATA4. The obtained results lay the foundation for future research in the framework of identifying the risks of developing cardiovascular changes in astronauts after space flights.
为了鉴定健康志愿者在头低位卧床 19 天后进行被动倾斜试验后血液蛋白质组的变化，对干血斑样本进行了色谱-质谱分析。结果表明，机体对倾斜试验的反应特征是高密度脂蛋白（HDL）和激肽原-1 的水平降低。倾斜试验后，我们观察到波形蛋白、维生素 K 依赖性蛋白 C、Wnt 信号通路蛋白、自噬和适应性免疫反应相关蛋白、黏着斑蛋白、血管损伤标志物 S100A8、PEDF 调节剂以及一些心脏蛋白：心肌肌动蛋白 ACTC1 和转录因子 GATA4 的水平增加。这些结果为未来在确定宇航员太空飞行后心血管变化风险的框架内进行研究奠定了基础。
