Department of Entomology, Texas A&M University, College station, TX, 77843, USA.
BMC Genomics. 2024 Feb 13;25(1):170. doi: 10.1186/s12864-024-10078-8.
This study explores the impact of disrupting the circadian clock through a Cycle gene knockout (KO) on the transcriptome of Aedes aegypti mosquitoes. The investigation aims to uncover the resulting alterations in gene expression patterns and physiological processes.
Transcriptome analysis was conducted on Cyc knockout (AeCyc) and wild-type mosquitoes at four time points in a light-dark cycle. The study identified system-driven genes that exhibit rhythmic expression independently of the core clock machinery. Cyc disruption led to altered expression of essential clock genes, affecting metabolic processes, signaling pathways, stimulus responses and immune responses. Notably, gene ontology enrichment of odorant binding proteins, indicating the clock's role in sensory perception. The absence of Cyc also impacted various regulation of metabolic and cell cycle processes was observed in all time points.
The intricate circadian regulation in Ae. aegypti encompasses both core clock-driven and system-driven genes. The KO of Cyc gene instigated extensive gene expression changes, impacting various processes, thereby potentially affecting cellular and metabolic functions, immune responses, and sensory perception. The circadian clock's multifaceted involvement in diverse biological processes, along with its role in the mosquito's daily rhythms, forms a nexus that influences the vector's capacity to transmit diseases. These insights shed light on the circadian clock's role in shaping mosquito biology and behavior, opening new avenues for innovative disease control strategies.
本研究通过敲除周期基因（Cycle gene knockout，KO）来扰乱生物钟，探讨其对埃及伊蚊转录组的影响。该研究旨在揭示基因表达模式和生理过程的变化。
在光照-黑暗周期的四个时间点，对 Cyc 敲除（AeCyc）和野生型蚊子进行了转录组分析。研究发现了系统驱动的基因，它们的表达节律独立于核心生物钟机制。Cyc 敲除导致必需生物钟基因的表达发生改变，影响代谢过程、信号通路、刺激反应和免疫反应。值得注意的是，气味结合蛋白的基因本体富集，表明时钟在感觉感知中的作用。在所有时间点，还观察到 Cyc 缺失对代谢和细胞周期过程的各种调节的影响。
埃及伊蚊复杂的昼夜节律调节包括核心时钟驱动和系统驱动的基因。Cyc 基因的 KO 引发了广泛的基因表达变化，影响了各种过程，从而可能影响细胞和代谢功能、免疫反应和感觉感知。生物钟在多种生物过程中的多方面参与，以及其在蚊子日常节律中的作用，形成了一个影响媒介传播疾病能力的节点。这些发现揭示了生物钟在塑造蚊子生物学和行为方面的作用，为创新的疾病控制策略开辟了新的途径。
