Insight into structural and chemical profile / composition of powdered enamel and dentine in different types of permanent human teeth.

Research on the structure and chemical composition of dental tissues allows for the optimisation of materials used in the treatment and care of teeth. Understanding pathological processes occurring in dental tissues and their reactions to various substances, including dental materials, are crucial for the development of new dental technologies. The aim of the study was to check the similarities in the chemical and morphological structure of enamel and dentine powders in various groups of permanent teeth, as well as differential chemical analysis for both grinded tissues tested. The extracted non-carious and non-pathological human permanent teeth were divided into four groups: incisors, canines, premolars and molars. Each tooth was sectioned to thick slices. Enamel and dentine were mechanically separated and ground in an agate mortar and pestle. FT-Raman and FTIR spectroscopy methods were used for the analysis of biological tissues. SEM method was applied to visualise hard dental tissues structures present on the surface and within the particles. The morphological structures were the same within the analysed tissues and did not depend on the analysed group of teeth. A comparison of the mineral-to-organic ratios of enamel and dentine in each tooth group showed that the bands related to PO were clearly higher in content for enamel than for dentine. Higher absorbance measured at the region of 2800-3700 cm and at 1500-1800 cm for dentine as compared to enamel samples were indicative of a higher content of organic structures. The highest contribution of phosphates was in canine enamel samples.The studies showed that the carbonate-to-phosphate ratio was higher for dentine (0.20 - 0.48) compared to the values obtained for enamel (0.13 - 0.22), however, minor differences were found in each group of enamel or dentine samples. The lack of significant differences between the enamel and dentine powders of incisors, canines, premolars and molars may prove that each extracted tooth, regardless of the tooth group, is an excellent substrate for their substitution.