f.a, sp. nov., a novel yeast species isolated from rotting wood in Brazil and Colombia.

Three yeast isolate candidates for a novel species were obtained from rotting wood samples collected in Brazil and Colombia. The Brazilian isolate differs from the Colombian isolates by one nucleotide substitution in each of the D1/D2 and small subunit (SSU) sequences. The internal transcribed spacer (ITS) and translation elongation factor 1-α gene sequences of the three isolates were identical. A phylogenetic analysis showed that this novel species belongs to the genus . This novel species is phylogenetically related to and . The novel species differs from by seven nucleotides and two indels, and by 17 nucleotides and four indels from in the D1/D2 sequences. The ITS sequences of these three species differ by more than 30 nucleotides. Analyses of the sequences of the SSU and translation elongation factor 1-α gene also showed that these isolates represent a novel species of the genus . Different from most species, these isolates did not assimilate methanol as the sole carbon source. The name sp. nov. is proposed to accommodate these isolates. The holotype of is CBS 13485. The MycoBank number is MB 851195.