College of Chinese Medicinal Materials, Jilin Agricultural University, 2888 Xincheng Street, Changchun , Jilin 130118, China.
Jilin Institute of Biology, 1244 Qianjin Street, Changchun, Jilin 130012, China.
J Chromatogr A. 2024 Mar 15;1718:464727. doi: 10.1016/j.chroma.2024.464727. Epub 2024 Feb 9.
In this study, we employed a melamine sponge (MS) as the skeleton material and utilized carbonized ZIF-8 (CZIF-8) and chitosan (CS) as the raw materials to prepare CZIF-8/CS-MS, a novel material featuring a three-dimensional interconnected porous network. The resulting CZIF-8/CS-MS material possesses a unique porous structure, significant specific surface area and abundant active sites. These characteristics make CZIF-8/CS-MS a promising absorbent for selective purification of plant growth regulators (PGRs) including 1-naphthlcetic acid (NAA), naphthoxyacetic acid (NOA), 4-chlorophenoxyacetic acid (4-CPA), 2,4-dichlorophenoxyacetic acid (2,4-D). After optimizing the extraction conditions, excellent linearity (r > 0.9994) was observed within a wide linear range of 1-100 ng/mL using ultra high performance liquid chromatography-tandem quadrupole mass spectrometry. The detection limits (LODs) and limits of quantification (LOQs) were found to be in the range of 0.013-0.154 ng/mL and 0.044-0.515 ng/mL, respectively. Additionally, the relative recovery of Schisandra chinensis fruit samples was determined to be 89.7-99.4 %, with a relative standard deviation (RSDs) of ≤ 8.4 % (n = 3). Compared to other methods, this approach offers a multitude of benefits, which include but are not limited to exceptional sensitivity, reduced sample volume requirements, low LODs, a comparable linear range, and high reproducibility. The findings of this study pave the way for exploring novel functionalized sponge columns, which leverage the integration of nano-sorbent materials and coating agents, for the purpose of analyzing PGRs within intricate matrix samples.
在这项研究中，我们采用三聚氰胺海绵（MS）作为骨架材料，利用碳化 ZIF-8（CZIF-8）和壳聚糖（CS）作为原料制备了具有三维互连通多孔网络结构的新型 CZIF-8/CS-MS 材料。所得 CZIF-8/CS-MS 材料具有独特的多孔结构、较大的比表面积和丰富的活性位点。这些特性使 CZIF-8/CS-MS 成为一种很有前途的吸附剂，可用于选择性纯化植物生长调节剂（PGRs），包括萘乙酸（NAA）、萘氧基乙酸（NOA）、4-氯苯氧乙酸（4-CPA）和 2,4-二氯苯氧乙酸（2,4-D）。在优化了提取条件后，利用超高效液相色谱-串联四极杆质谱法，在 1-100ng/mL 的较宽线性范围内观察到极好的线性（r＞0.9994）。检测限（LOD）和定量限（LOQ）分别为 0.013-0.154ng/mL 和 0.044-0.515ng/mL。此外，五味子果实样品的相对回收率为 89.7-99.4％，相对标准偏差（RSDs）≤8.4％（n=3）。与其他方法相比，该方法具有许多优势，包括但不限于出色的灵敏度、较少的样品体积需求、较低的检测限、可比的线性范围和较高的重现性。本研究为探索新型功能化海绵柱奠定了基础，该海绵柱利用纳米吸附材料和涂层剂的整合，用于分析复杂基质样品中的 PGRs。
