Ebrahimibarogh Robab, Ghazanfari Mona, Shokohi Tahereh, Yousefiabdolmaleki Ensieh, Seyedmousavi Amir, Hedayati Mohammad T
Student Research Committee Center, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Med Mycol Case Rep. 2024 Feb 1;43:100629. doi: 10.1016/j.mmcr.2024.100629. eCollection 2024 Mar.
Tinea capitis is a chronic fungal infection of the scalp occurring commonly in children of school age, especially in developing countries. It is caused primarily by the dermatophyte members of genera and . Here we report presence of free-living mycelial stage of dermatophytes, a stage of fungal growth which form in culture medias, around affected hairs and skin scrapings of scalp lesions in a 3-year-old boy presenting with alopecia and multiple scaly, non-erythematous plaques. On direct microscopy examination using 10 % potassium hydroxide, the fungal hyphae and arthrospores were detected in ectothrix form. In addition, we also observed numerous multicellular, thick-walled spindle-shaped macroconidia around hairs and skin scrapings. To our knowledge this is the first study reporting dermatophyte's macroconidia directly seen on clinical samples. Species level identification of the dermatophyte isolate growing on Mycosel™ agar was confirmed by PCR-sequencing of internal transcribed spacer of ribosomal RNA as The patient was successfully treated with systemic itraconazole combined with topical ketoconazole shampoo.
头癣是一种常见于学龄儿童的头皮慢性真菌感染，在发展中国家尤为常见。它主要由 和 属的皮肤癣菌引起。在此，我们报告了在一名3岁男孩的头皮病变毛发和皮肤刮屑周围发现了皮肤癣菌的自由生活菌丝体阶段，这是真菌在培养基中形成的生长阶段，该男孩表现为脱发以及多个鳞屑性、非红斑性斑块。在使用10%氢氧化钾进行直接显微镜检查时，检测到了外发癣菌形式的真菌菌丝和关节孢子。此外，我们还在毛发和皮肤刮屑周围观察到大量多细胞、厚壁纺锤形大分生孢子。据我们所知，这是第一项直接在临床样本上观察到皮肤癣菌大分生孢子的研究。通过对核糖体RNA内部转录间隔区进行PCR测序，确认在Mycosel™琼脂上生长的皮肤癣菌分离株在种水平上为 。该患者通过口服伊曲康唑联合外用酮康唑洗发水成功治愈。
