Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Rodovia Celso Garcia Cid PO-BOX 6001, Londrina, 86051-980, Brazil.
Laboratory of Microbial Biotechnology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil.
Curr Microbiol. 2024 Feb 19;81(4):100. doi: 10.1007/s00284-024-03616-w.
This study aimed to assess the activity of AgNPs biosynthesized by Fusarium oxysporum (bio-AgNPs) against multidrug-resistant uropathogenic Proteus mirabilis, and to assess the antibacterial activity of catheters coated with bio-AgNPs. Broth microdilution and time-kill kinetics assays were used to determine the antibacterial activity of bio-AgNPs. Catheters were coated with two (2C) and three (3C) bio-AgNPs layers using polydopamine as crosslinker. Catheters were challenged with urine inoculated with P. mirabilis to assess the anti-incrustation activity. MIC was found to be 62.5 µmol l-1, causing total loss of viability after 4 h and bio-AgNPs inhibited biofilm formation by 76.4%. Catheters 2C and 3C avoided incrustation for 13 and 20 days, respectively, and reduced biofilm formation by more than 98%, while the pristine catheter was encrusted on the first day. These results provide evidence for the use of bio-AgNPs as a potential alternative to combat of multidrug-resistant P. mirabilis infections.
本研究旨在评估由尖孢镰刀菌（bio-AgNPs）生物合成的银纳米粒子（AgNPs）对多药耐药尿路致病性奇异变形杆菌的活性，并评估涂有 bio-AgNPs 的导管的抗菌活性。采用肉汤微量稀释法和时间杀伤动力学试验来测定 bio-AgNPs 的抗菌活性。使用聚多巴胺作为交联剂，将导管涂覆两层（2C）和三层（3C）bio-AgNPs。用接种奇异变形杆菌的尿液对导管进行挑战，以评估抗结垢活性。MIC 为 62.5µmol·l-1，4 小时后导致完全丧失活力，bio-AgNPs 抑制生物膜形成 76.4%。导管 2C 和 3C 分别避免结垢 13 天和 20 天，生物膜形成减少 98%以上，而原始导管在第一天就结垢。这些结果为使用 bio-AgNPs 作为对抗多药耐药奇异变形杆菌感染的潜在替代物提供了证据。
