一种用于测定变形链球菌和乳酸杆菌感染的微量方法的评估。
Evaluation of a micromethod for determination of Streptococcus mutans and Lactobacillus infection.
作者信息
Westergren G, Krasse B
出版信息
J Clin Microbiol. 1978 Jan;7(1):82-3. doi: 10.1128/jcm.7.1.82-83.1978.
Abstract
A micromethod was developed for quantitative estimation in saliva of Streptococcus mutans and lactobacilli. With a semiautomatic pipette, 25 microliter of diluted saliva was spotted on the surface of an agar plate containing a selective medium. This volume gave a spot with a diameter of about 10 mm in which separate colonies could be counted. The results obtained with the spotting technique showed excellent agreement with those obtained with conventional agar plating. The method is convenient and results in a substantial saving of culture media.
摘要
开发了一种微量方法用于定量评估唾液中的变形链球菌和乳酸菌。使用半自动移液器，将25微升稀释后的唾液点样在含有选择性培养基的琼脂平板表面。这个体积的唾液形成的斑点直径约为10毫米，在其中可以计数单个菌落。斑点技术得到的结果与传统琼脂平板培养法得到的结果高度一致。该方法方便且可大幅节省培养基。
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