Department of Biostatistics, Faculty of Veterinary Medicine, Balikesir University, Balikesir, Turkey.
Department of Animal Science, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey.
Vet Med Sci. 2024 Mar;10(2):e1387. doi: 10.1002/vms3.1387.
In ovo application is the process of administering some nutrients or components into the egg. The main purpose of this application is to ensure that some nutrients are provided to chicks with a short incubation period. Few studies were conducted with taurine in fertile eggs; especially, no observation of hatchability and chick quality has been found. In addition, taurine has an anti-stress impact that fights oxidative factors.
To assess the hatchability and chick quality after in ovo taurine administration. To determine the stress that may occur as a result of in ovo application and whether taurine has a stress-reducing effect.
A total of 1200 fertile eggs from a 34-week-old broiler breeder (Ross 308) flock were categorized into 4 groups with 75 eggs per replicate: control (uninjected), taurine group (0.30 mL dissolved taurine in distilled water), sham control (sterile distilled water) and perforation (eggs perforated and then waxed). On day 14 of incubation, an in ovo injection was administered to the albumen. Data on hatching parameters and hepatic HSP70 levels were obtained using relevant formulas and western blotting, respectively.
Control chicks exhibited higher hatchability than other groups, with the taurine group showing the lowest hatchability. The HSP70 levels were the highest in the perforation group compared to the control group. An increase of 21.37% in the taurine group and 83.45% in the sham control group was observed compared to the control group.
The findings suggest that in ovo application may induce increased stress, whereas taurine may have positive effects in mitigating the stress caused by in ovo application.
在鸡胚中应用是将某些营养物质或成分注入鸡蛋的过程。这种应用的主要目的是确保一些营养物质能够提供给孵化期较短的小鸡。在可育蛋中很少有关于牛磺酸的研究；特别是，没有发现孵化率和雏鸡质量的观察结果。此外，牛磺酸具有抗应激作用，可以对抗氧化因素。
评估在鸡胚中给予牛磺酸后的孵化率和雏鸡质量。确定由于在鸡胚中应用而可能发生的应激以及牛磺酸是否具有减轻应激的作用。
从 34 周龄的肉鸡种鸡（罗斯 308）群中收集了 1200 枚可育蛋，分为 4 组，每组 75 枚重复：对照组（未注射）、牛磺酸组（0.30 毫升溶解在蒸馏水中的牛磺酸）、假对照组（无菌蒸馏水）和穿孔组（鸡蛋穿孔后涂蜡）。在孵化的第 14 天，对蛋白进行了在鸡胚中的注射。使用相关公式和 Western blot 分别获得孵化参数和肝 HSP70 水平的数据。
对照组雏鸡的孵化率高于其他组，牛磺酸组的孵化率最低。穿孔组的 HSP70 水平与对照组相比最高。与对照组相比，牛磺酸组增加了 21.37%，假对照组增加了 83.45%。
研究结果表明，在鸡胚中应用可能会引起应激增加，而牛磺酸可能具有减轻在鸡胚中应用引起的应激的积极作用。
