重金属和糖皮质激素对小鼠金属硫蛋白I和II基因的协同调控
Coordinate regulation of mouse metallothionein I and II genes by heavy metals and glucocorticoids.
作者信息
Yagle M K, Palmiter R D
出版信息
Mol Cell Biol. 1985 Feb;5(2):291-4. doi: 10.1128/mcb.5.2.291-294.1985.
Abstract
Regulation of the endogenous mouse metallothionein I and II (MT-I and MT-II) genes by heavy metals and glucocorticoids was studied in cultured mouse cells. Both mRNAs were measured simultaneously by solution hybridization with [3H]MT-I cDNA and [32P]MT-II cDNA, and the absolute amount of each mRNA was calculated by using a single-stranded M13 standard that contained both mRNA sequences. Both genes responded identically to different concentrations of metals (zinc, cadmium, and copper) and dexamethasone. Furthermore, the time courses of induction of both mRNAs were the same. However, under all conditions there was 1.2- to 1.9-fold more MT-I mRNA than MT-II mRNA. We conclude that both genes are regulated identically by receptors for glucocorticoids and metals but that the rate of transcription from the MT-I gene is slightly higher than from the MT-II gene.
摘要
在培养的小鼠细胞中研究了重金属和糖皮质激素对内源小鼠金属硫蛋白I和II（MT-I和MT-II）基因的调控。通过与[3H]MT-I cDNA和[32P]MT-II cDNA进行溶液杂交同时测量两种mRNA，并使用包含两种mRNA序列的单链M13标准物计算每种mRNA的绝对量。两种基因对不同浓度的金属（锌、镉和铜）和地塞米松的反应相同。此外，两种mRNA诱导的时间进程相同。然而，在所有条件下，MT-I mRNA比MT-II mRNA多1.2至1.9倍。我们得出结论，两种基因受糖皮质激素和金属受体的调控方式相同，但MT-I基因的转录速率略高于MT-II基因。
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