用于测定风疹抗体的商业酶联免疫吸附测定法（Rubazyme）与凝胶内溶血试验的比较。
Comparison between a commercial ELISA, Rubazyme, and hemolysis-in-gel test for determination of rubella antibodies.
作者信息
Grillner L, Forsgren M, Nordenfelt E
出版信息
J Virol Methods. 1985 Feb;10(2):111-5. doi: 10.1016/0166-0934(85)90096-5.
A commercial enzyme-linked immunosorbent assay, Rubazyme, was compared with the hemolysis-in-gel (HIG) test for antibodies to rubella in 826 sera. The results were in agreement for 99.4% of the 725 sera tested for immunity. However, the Rubazyme assay was no more efficient that either the hemagglutination-inhibition or HIG test in discriminating between sera with low levels of antibody and negative sera. Thus, it was concluded that the HIG test is the method of choice for immunity testing because of the low cost and simplicity. Rubazyme may be of value to confirm equivocal HIG results.
采用商业酶联免疫吸附试验（Rubazyme）与凝胶内溶血试验（HIG）对826份血清中的风疹抗体进行检测，并比较二者结果。在725份检测免疫性的血清中，99.4%的结果一致。然而，在区分低水平抗体血清和阴性血清方面，Rubazyme试验并不比血凝抑制试验或HIG试验更有效。因此，得出结论：由于成本低且操作简单，HIG试验是免疫检测的首选方法。Rubazyme可能有助于确认HIG结果不明确的情况。
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