Frazer Institute, The University of Queensland, Brisbane, QLD, Australia.
The Centenary Institute, Sydney, NSW, Australia.
Methods Mol Biol. 2024;2764:291-310. doi: 10.1007/978-1-0716-3674-9_19.
Aberrant cell cycle progression is a hallmark of solid tumors. Therefore, cell cycle analysis is an invaluable technique to study cancer cell biology. However, cell cycle progression has been most commonly assessed by methods that are limited to temporal snapshots or that lack spatial information. In this chapter, we describe a technique that allows spatiotemporal real-time tracking of cell cycle progression of individual cells in a multicellular context. The power of this system lies in the use of 3D melanoma spheroids generated from melanoma cells engineered with the fluorescent ubiquitination-based cell cycle indicator (FUCCI). This technique, combined with mathematical modeling, allows us to gain further and more detailed insight into several relevant aspects of solid cancer cell biology, such as tumor growth, proliferation, invasion, and drug sensitivity.
细胞周期异常是实体瘤的一个标志。因此，细胞周期分析是研究癌细胞生物学的一种非常有价值的技术。然而，细胞周期的进展通常是通过只能提供时间快照或缺乏空间信息的方法来评估的。在这一章中，我们描述了一种技术，该技术允许在多细胞环境中实时跟踪单个细胞的细胞周期进展的时空轨迹。该系统的优势在于使用 3D 黑色素瘤球体，这些球体是由经过荧光泛素化细胞周期指示剂 (FUCCI) 工程改造的黑色素瘤细胞生成的。该技术与数学建模相结合，使我们能够更深入、更详细地了解实体癌细胞生物学的几个相关方面，如肿瘤生长、增殖、侵袭和药物敏感性。
