海洋细菌蛋白酶VSP2V-280对鱼小清蛋白的高效水解：过敏原去除
Efficient Hydrolysis of Fish Parvalbumin by Marine Bacterial Protease VSP2V-280: Allergen Removal.
作者信息
Zhou Junlan, Bai Yue, Gao Yuan, Tian Huili, Wang Ming'ao, Kang Xinxin, Zhang Lei, Lv Mingsheng, Wang Shujun
机构信息
Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology Jiangsu Ocean University Lianyungang China.
Co-Innovation Center of Jiangsu Marine bio-Industry Technology Jiangsu Ocean University Lianyungang China.
出版信息
Food Sci Nutr. 2025 Jan 7;13(1):e4729. doi: 10.1002/fsn3.4729. eCollection 2025 Jan.
Parvalbumin is a major allergen in fish. However, there is currently no effective and safe way to remove this allergen from fish. In this study, protease gene VSP2V-280 of marine bacteria sp. SP2 was cloned and expressed. The protease enzyme showed maximum activity at 50°C and pH 10.0. Ca and Cu promoted the enzyme. The enzyme showed good parvalbumin degradation efficiency in fish. Based on the gel analysis, when 0.3 mg/mL of parvalbumin was incubated with protease VSP2V-280 (30 U/mL) containing 1 mM Ca for 3 h, the parvalbumin removal rate reached 97%. The enzyme was further used for parvalbumin removal from , , , and . The parvalbumin removal rate reached 93% in 4 h at an enzyme dosage of 72 U/mL. The study showed the potential of VSP2V-280 to remove parvalbumin from aquatic products.
小清蛋白是鱼类中的一种主要过敏原。然而，目前尚无有效且安全的方法从鱼类中去除这种过敏原。在本研究中，克隆并表达了海洋细菌sp. SP2的蛋白酶基因VSP2V - 280。该蛋白酶在50°C和pH 10.0时表现出最大活性。钙和铜可促进该酶的活性。该酶在鱼类中对小清蛋白具有良好的降解效率。基于凝胶分析，当0.3 mg/mL的小清蛋白与含有1 mM钙的蛋白酶VSP2V - 280（30 U/mL）孵育3小时时，小清蛋白去除率达到97%。该酶进一步用于从[此处原文缺失具体鱼类名称]、[此处原文缺失具体鱼类名称]、[此处原文缺失具体鱼类名称]和[此处原文缺失具体鱼类名称]中去除小清蛋白。在酶剂量为72 U/mL时，4小时内小清蛋白去除率达到93%。该研究表明VSP2V - 280具有从水产品中去除小清蛋白的潜力。
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