• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

海洋细菌蛋白酶VSP2V-280对鱼小清蛋白的高效水解：过敏原去除

Efficient Hydrolysis of Fish Parvalbumin by Marine Bacterial Protease VSP2V-280: Allergen Removal.

作者信息

Zhou Junlan, Bai Yue, Gao Yuan, Tian Huili, Wang Ming'ao, Kang Xinxin, Zhang Lei, Lv Mingsheng, Wang Shujun

机构信息

Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology Jiangsu Ocean University Lianyungang China.

Co-Innovation Center of Jiangsu Marine bio-Industry Technology Jiangsu Ocean University Lianyungang China.

出版信息

Food Sci Nutr. 2025 Jan 7;13(1):e4729. doi: 10.1002/fsn3.4729. eCollection 2025 Jan.

DOI:10.1002/fsn3.4729
PMID:39803265
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11717067/
Abstract

Parvalbumin is a major allergen in fish. However, there is currently no effective and safe way to remove this allergen from fish. In this study, protease gene VSP2V-280 of marine bacteria sp. SP2 was cloned and expressed. The protease enzyme showed maximum activity at 50°C and pH 10.0. Ca and Cu promoted the enzyme. The enzyme showed good parvalbumin degradation efficiency in fish. Based on the gel analysis, when 0.3 mg/mL of parvalbumin was incubated with protease VSP2V-280 (30 U/mL) containing 1 mM Ca for 3 h, the parvalbumin removal rate reached 97%. The enzyme was further used for parvalbumin removal from , , , and . The parvalbumin removal rate reached 93% in 4 h at an enzyme dosage of 72 U/mL. The study showed the potential of VSP2V-280 to remove parvalbumin from aquatic products.

摘要

小清蛋白是鱼类中的一种主要过敏原。然而，目前尚无有效且安全的方法从鱼类中去除这种过敏原。在本研究中，克隆并表达了海洋细菌sp. SP2的蛋白酶基因VSP2V - 280。该蛋白酶在50°C和pH 10.0时表现出最大活性。钙和铜可促进该酶的活性。该酶在鱼类中对小清蛋白具有良好的降解效率。基于凝胶分析，当0.3 mg/mL的小清蛋白与含有1 mM钙的蛋白酶VSP2V - 280（30 U/mL）孵育3小时时，小清蛋白去除率达到97%。该酶进一步用于从[此处原文缺失具体鱼类名称]、[此处原文缺失具体鱼类名称]、[此处原文缺失具体鱼类名称]和[此处原文缺失具体鱼类名称]中去除小清蛋白。在酶剂量为72 U/mL时，4小时内小清蛋白去除率达到93%。该研究表明VSP2V - 280具有从水产品中去除小清蛋白的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/afb47ddd9832/FSN3-13-e4729-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/dfa63a7a6a21/FSN3-13-e4729-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/f0016d830153/FSN3-13-e4729-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/bdcf7bdc01f8/FSN3-13-e4729-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/d0a992ad9bb7/FSN3-13-e4729-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/9515905f1770/FSN3-13-e4729-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/c6c43ffe2eca/FSN3-13-e4729-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/9c3ae4fbb069/FSN3-13-e4729-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/afb47ddd9832/FSN3-13-e4729-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/dfa63a7a6a21/FSN3-13-e4729-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/f0016d830153/FSN3-13-e4729-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/bdcf7bdc01f8/FSN3-13-e4729-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/d0a992ad9bb7/FSN3-13-e4729-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/9515905f1770/FSN3-13-e4729-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/c6c43ffe2eca/FSN3-13-e4729-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/9c3ae4fbb069/FSN3-13-e4729-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1934/11717067/afb47ddd9832/FSN3-13-e4729-g001.jpg

相似文献

1
Efficient Hydrolysis of Fish Parvalbumin by Marine Bacterial Protease VSP2V-280: Allergen Removal.海洋细菌蛋白酶VSP2V-280对鱼小清蛋白的高效水解：过敏原去除
Food Sci Nutr. 2025 Jan 7;13(1):e4729. doi: 10.1002/fsn3.4729. eCollection 2025 Jan.
2
Molecular and immunological characterization of grass carp (Ctenopharyngodon idella) parvalbumin Cten i 1: A major fish allergen in Hong Kong.草鱼（Ctenopharyngodon idella）副肌球蛋白 Cten i 1 的分子和免疫特性：香港的一种主要鱼类过敏原。
Pediatr Allergy Immunol. 2020 Oct;31(7):792-804. doi: 10.1111/pai.13259. Epub 2020 Jun 26.
3
Microhomology-mediated end-joining Knock-In approaches to delete the allergenic domain of trout . Preliminary results in F0 animals and feedback.通过微同源性介导的末端连接敲入方法删除鳟鱼的过敏原结构域。F0代动物的初步结果及反馈。
Open Res Eur. 2025 Feb 25;4:235. doi: 10.12688/openreseurope.18223.2. eCollection 2024.
4
Exploring Fish Parvalbumins through Allergen Names and Gene Identities.通过过敏原名称和基因身份探索鱼类 parvalbumins。
Genes (Basel). 2024 Oct 18;15(10):1337. doi: 10.3390/genes15101337.
5
Characterization of detergent compatible protease from halophilic sp. CD6.嗜盐菌CD6中与去污剂兼容的蛋白酶的特性分析
3 Biotech. 2018 Feb;8(2):104. doi: 10.1007/s13205-018-1133-2. Epub 2018 Jan 31.
6
Quantification of major allergen parvalbumin in 22 species of fish by SDS-PAGE.用 SDS-PAGE 定量分析 22 种鱼类中的主要过敏原 parvalbumin。
Food Chem. 2016 Mar 1;194:345-53. doi: 10.1016/j.foodchem.2015.08.037. Epub 2015 Aug 12.
7
Cross-reactive monoclonal antibodies against fish parvalbumins as a tool for studying antigenic similarity of different parvalbumins and analysis of fish extracts.针对鱼类副肌球蛋白的交叉反应性单克隆抗体作为研究不同副肌球蛋白抗原相似性和鱼类提取物分析的工具。
Mol Immunol. 2023 Feb;154:80-95. doi: 10.1016/j.molimm.2023.01.001. Epub 2023 Jan 6.
8
Antibody reactivity to the major fish allergen parvalbumin is determined by isoforms and impact of thermal processing.抗体对主要鱼类过敏原副肌球蛋白的反应性由同工型决定，并受热加工的影响。
Food Chem. 2014 Apr 1;148:321-8. doi: 10.1016/j.foodchem.2013.10.035. Epub 2013 Oct 18.
9
Cloning of two distinct cDNAs encoding parvalbumin, the major allergen of Atlantic salmon (Salmo salar).两种编码小白蛋白（大西洋鲑鱼（Salmo salar）的主要过敏原）的不同cDNA的克隆。
Scand J Immunol. 1996 Oct;44(4):335-44. doi: 10.1046/j.1365-3083.1996.d01-314.x.
10
Protein and DNA-based assays as complementary tools for fish allergen detection.基于蛋白质和DNA的检测方法作为鱼类过敏原检测的互补工具。
Allergol Select. 2017 Aug 4;1(2):120-126. doi: 10.5414/ALX01485E. eCollection 2017.

本文引用的文献

1
3D bio-printing-based vascular-microtissue electrochemical biosensor for fish parvalbumin detection.基于 3D 生物打印的血管微组织电化学生物传感器用于鱼类 parvalbumin 的检测。
Food Chem. 2024 Jul 1;445:138799. doi: 10.1016/j.foodchem.2024.138799. Epub 2024 Feb 18.
2
Calcium-binding protein parvalbumin in the spinal cord and dorsal root ganglia.脊髓和背根神经节中的钙结合蛋白 parvalbumin。
Neurochem Int. 2023 Dec;171:105634. doi: 10.1016/j.neuint.2023.105634. Epub 2023 Nov 13.
3
Prevalence of self-reported food allergy in Tunisia: General trends and probabilistic modeling.
突尼斯自我报告的食物过敏患病率：总体趋势和概率建模。
World Allergy Organ J. 2023 Sep 28;16(9):100813. doi: 10.1016/j.waojou.2023.100813. eCollection 2023 Sep.
4
Food allergy prevention: Where are we in 2023?食物过敏预防：2023年我们处于什么阶段？
Asia Pac Allergy. 2023 Mar;13(1):15-27. doi: 10.5415/apallergy.0000000000000001. Epub 2023 Apr 28.
5
An update on recent developments and highlights in food allergy.食物过敏的最新进展和重点更新。
Allergy. 2023 Sep;78(9):2344-2360. doi: 10.1111/all.15749. Epub 2023 May 12.
6
IgE-immunoadsorption for severe allergy to multiple foods: A case series of five children.IgE免疫吸附治疗对多种食物严重过敏：5例儿童病例系列
World Allergy Organ J. 2023 Feb 15;16(2):100750. doi: 10.1016/j.waojou.2023.100750. eCollection 2023 Feb.
7
Parvalbumin: A Major Fish Allergen and a Forensically Relevant Marker.副肌球蛋白：一种主要的鱼类过敏原和法医学相关标志物。
Genes (Basel). 2023 Jan 14;14(1):223. doi: 10.3390/genes14010223.
8
Effect of species, muscle location, food processing and refrigerated storage on the fish allergens, tropomyosin and parvalbumin.不同物种、肌肉部位、食品加工和冷藏储存对鱼类过敏原、肌球蛋白轻链和副肌球蛋白的影响。
Food Chem. 2023 Feb 15;402:134479. doi: 10.1016/j.foodchem.2022.134479. Epub 2022 Oct 1.
9
Detection of Parvalbumin Fish Allergen in Canned Tuna by Real-Time PCR Driven by Tuna Species and Can-Filling Medium.实时 PCR 检测金枪鱼罐头中副肌球蛋白过敏原：基于金枪鱼种类和罐头填充介质的驱动。
Molecules. 2022 Sep 2;27(17):5674. doi: 10.3390/molecules27175674.
10
A brief history of allergen immunotherapy.变应原免疫治疗的简史。
Allergy Asthma Proc. 2022 Jul 1;43(4):248-253. doi: 10.2500/aap.2022.43.220023.