Wu Zhanwei, Luo Lu, Wan Yongshan, Liu Fengzhen
State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
College of Agronomy, Shandong Agricultural University, Tai'an, China.
Front Plant Sci. 2023 Jan 27;14:1093913. doi: 10.3389/fpls.2023.1093913. eCollection 2023.
Plant protein phosphatase 2C (PP2C) play important roles in response to salt stress by influencing metabolic processes, hormone levels, growth factors, etc. Members of the PP2C family have been identified in many plant species. However, they are rarely reported in peanut. In this study, 178 genes were identified in peanut, which were unevenly distributed across the 20 chromosomes, with segmental duplication in 78 gene pairs. AhPP2Cs could be divided into 10 clades (A-J) by phylogenetic analysis. s had experienced segmental duplications and strong purifying selection pressure. 22 miRNAs from 14 different families were identified, targeting 57 genes. Gene structures and motifs analysis exhibited s in subclades AI and AII had high structural and functional similarities. Phosphorylation sites of ///// were predicted in motifs 2 and 4, which located within the catalytic site at the C-terminus. We discovered multiple MYB binding factors and ABA response elements in the promoter regions of the six genes (/////) by -elements analysis. GO and KEGG enrichment analysis confirmed genes in protein binding, signal transduction, protein modification process response to abiotic stimulus through environmental information processing. Based on RNA-Seq data of 22 peanut tissues, clade A s showed a varying degree of tissue specificity, of which, and specifically expressed in seeds, while /// expressed in leaves and roots. qRT-PCR indicated that and displayed significantly up-regulated expression in response to salt stress. These results indicated that and could be candidate conferring salt tolerance. These results provide further insights into the peanut gene family and indicate potentially involved in the response to salt stress, which can now be further investigated in peanut breeding efforts to obtain cultivars with improved salt tolerance.
植物蛋白磷酸酶2C（PP2C）通过影响代谢过程、激素水平、生长因子等在盐胁迫响应中发挥重要作用。PP2C家族成员已在许多植物物种中被鉴定出来。然而，它们在花生中鲜有报道。在本研究中，在花生中鉴定出178个基因，这些基因不均匀地分布在20条染色体上，78个基因对存在片段重复。通过系统发育分析，AhPP2Cs可分为10个进化枝（A - J）。它们经历了片段重复和强烈的纯化选择压力。鉴定出14个不同家族的22个miRNA，靶向57个基因。基因结构和基序分析表明，进化枝AI和AII中的基因具有高度的结构和功能相似性。在位于C端催化位点内的基序2和4中预测了/////的磷酸化位点。通过元件分析，我们在六个基因（/////）的启动子区域发现了多个MYB结合因子和ABA响应元件。GO和KEGG富集分析证实，这些基因通过环境信息处理参与蛋白质结合、信号转导、蛋白质修饰过程以响应非生物刺激。基于22个花生组织的RNA - Seq数据，进化枝A中的基因表现出不同程度的组织特异性，其中，和在种子中特异性表达，而///在叶和根中表达。qRT - PCR表明，和在盐胁迫下表现出显著上调的表达。这些结果表明，和可能是赋予耐盐性的候选基因。这些结果为花生基因家族提供了进一步的见解，并表明其可能参与盐胁迫响应，现在可以在花生育种工作中进一步研究以获得耐盐性提高的品种。
