State Key Laboratory of Aridland Crop Science, College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Institute of Cotton Research, State Key Laboratory of Cotton Biology, Chinese Academy of Agricultural Sciences (CAAS), Anyang 455000, China.
Int J Mol Sci. 2024 Feb 4;25(3):1877. doi: 10.3390/ijms25031877.
Annexins (ANNs) are a structurally conserved protein family present in almost all plants. In the present study, 27 were identified in cotton and were unevenly distributed across 14 chromosomes. Transcriptome data and RT-qPCR results revealed that multiple respond to at least two abiotic stresses. Similarly, the expression levels of and were significantly upregulated under heat, cold, and drought stress. Using virus-induced gene silencing (VIGS), functional characterization of and revealed that, compared with those of the controls, the leaf wilting of -silenced plants was more obvious, and the activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were lower under NaCl and PEG stress. Moreover, the expression of stress marker genes (, , , , , , , , , and ) was significantly downregulated in -silenced plants after stress. Under cold stress, the growth of the -silenced plants was significantly weaker than that of the control plants, and the activities of POD, SOD, and CAT were also lower. However, compared with those of the control, the elasticity and orthostatic activity of the -silenced plants were greater; the POD, SOD, and CAT activities were higher; and the , , and expression levels were greater under heat stress. These results suggest that different family members respond differently to different types of abiotic stress.
膜联蛋白（ANNs）是一种结构保守的蛋白质家族，几乎存在于所有植物中。在本研究中，在棉花中鉴定出了 27 种，它们不均匀地分布在 14 条染色体上。转录组数据和 RT-qPCR 结果表明，多种 对至少两种非生物胁迫有反应。同样，在热、冷和干旱胁迫下， 和 的表达水平显著上调。通过病毒诱导的基因沉默（VIGS）， 和 的功能表征表明，与对照相比，沉默植株的叶片萎蔫更为明显，CAT（过氧化氢酶）、POD（过氧化物酶）和 SOD（超氧化物歧化酶）的活性在 NaCl 和 PEG 胁迫下较低。此外，在胁迫后，沉默植株中应激标记基因（ 、 、 、 、 、 、 、 、 和 ）的表达明显下调。在冷胁迫下，沉默植株的生长明显弱于对照植株，POD、SOD 和 CAT 的活性也较低。然而，与对照相比，沉默植株的弹性和直立活动能力更强；POD、SOD 和 CAT 的活性更高；在热胁迫下， 、 、 和 的表达水平更高。这些结果表明，不同的 家族成员对不同类型的非生物胁迫有不同的反应。
