Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Biotechnol Lett. 2021 May;43(5):1089-1102. doi: 10.1007/s10529-021-03110-4. Epub 2021 Mar 10.
To study the possible roles of type-2C protein phosphatases (PP2Cs) which have been confirmed to play roles in the response to diverse abiotic stresses in paper mulberry, we launched a series of genomic and functional studies of BpPP2Cs.
Sixty-three PP2C proteins in paper mulberry (Broussonetia papyrifera) were classified into 13 clades. Four BpPP2Cs with kinase domains were verified to be highly conserved in organisms ranging from algae to dicots. Seven pairs of BpPP2C genes were found to be expanding, and 18 BpPP2C genes had orthologues in Arabidopsis. BpPP2Cs showed broad expression in different tissues; the expression levels of 18 BpPP2Cs were changed and the phosphorylation levels of seven BpPP2C proteins increased at low temperature. Cold-response elements were found in the promoter region of 31 BpPP2Cs. Finally, Bp01g0320 was found to act as a hub protein and Bp01g0512 and Bp09g1278 played key roles in the ABA-signaling pathway and MAPK cascades, respectively.
These results suggest that the PP2C gene family of paper mulberry is evolutionarily conserved and participates the regulation of the response to cold stress, which will play a vital role in further research on phosphatases in paper mulberry.
研究已证实参与多种非生物胁迫响应的 2C 型蛋白磷酸酶（PP2Cs）在构树中的可能作用，我们对 BpPP2Cs 进行了一系列基因组和功能研究。
构树（Broussonetia papyrifera）中的 63 种 PP2C 蛋白被分为 13 个分支。四个具有激酶结构域的 BpPP2C 被证实高度保守，存在于从藻类到双子叶植物的生物中。发现 7 对 BpPP2C 基因在扩张，18 个 BpPP2C 基因在拟南芥中有同源物。BpPP2Cs 在不同组织中广泛表达；18 个 BpPP2C 的表达水平发生变化，7 个 BpPP2C 蛋白的磷酸化水平在低温下升高。在 31 个 BpPP2Cs 的启动子区域发现了冷响应元件。最后，发现 Bp01g0320 作为一个枢纽蛋白，Bp01g0512 和 Bp09g1278 分别在 ABA 信号通路和 MAPK 级联中起关键作用。
这些结果表明，构树的 PP2C 基因家族在进化上是保守的，参与了对冷胁迫的调控，这将在构树中进一步研究磷酸酶方面发挥重要作用。
