Suhendro Ikhsan, Rachman Noor Ronny, Jakaria Jakaria, Priyanto Rudy, Manalu Wasmen, Andersson Göran
Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor 16680, Indonesia.
Department of Animal Science, Tulang Bawang University, Bandar Lampung 35121, Indonesia.
Vet World. 2024 Jan;17(1):17-25. doi: 10.14202/vetworld.2024.17-25. Epub 2024 Jan 4.
Global warming challenges cattle productivity and welfare since it affects heat stress and scarce feed. The heat-shock protein 70 () gene is essential in cytoprotection against stressors, protecting cells from dysregulated gene expression and apoptosis. This study aimed to identify significant genetic markers of the gene that can be leveraged genetically to enhance thermotolerance and production in Bali cattle further.
Animals were sampled from three different rearing systems. In this study, 83 healthy adult male Bali cattle without abnormalities were utilized. Single-nucleotide polymorphism (SNP) diversity associated with the physiological and physical traits of Bali cattle was assessed using SNPStat online software. Gene expression for putative SNPs and their genotypic groups was further evaluated.
There were 15 polymorphic SNPs (c.-185G>A, c.-69T>G, c.10G>C, c.19A>G, c.45C>T, c.101INS, c.115T>C, c.130T>C, c.136G>T, c.159G>C, c.164G>T, c.234G>A, c.303G>A, c.333C>A, and c.456C>T) identified, of which 12 were associated with the assessed trait. Nine SNPs were associated with physiological traits, while eight were with physical traits. The c.136G>T as a novel, high minor allele frequency, and associative SNP was selected for gene expression. Individuals with the TT genotype have a trim physique, susceptible physiology, and high mRNA expression. On the other hand, the GG genotype was significantly associated with larger physique, lower physiology, and low mRNA expression. The higher expression may indicate that is involved in mitigating the deleterious effects of stress. As a result, the animal experienced negative energy balance, decreasing body size.
Single-nucleotide polymorphism c.136G>T is a candidate biomarker for heat resistance traits in Bali cattle.
全球变暖对牛的生产力和福利构成挑战，因为它会影响热应激和饲料短缺问题。热休克蛋白70（）基因在细胞对应激源的保护中至关重要，可保护细胞免受基因表达失调和细胞凋亡的影响。本研究旨在确定该基因的重要遗传标记，以便通过基因手段进一步提高巴厘牛的耐热性和生产性能。
从三种不同的饲养系统中采集动物样本。在本研究中，使用了83头无异常的健康成年雄性巴厘牛。使用SNPStat在线软件评估与巴厘牛生理和身体特征相关的单核苷酸多态性（SNP）多样性。进一步评估推定SNP及其基因型组的基因表达。
共鉴定出15个多态性SNP（c.-185G>A、c.-69T>G、c.10G>C、c.19A>G、c.45C>T、c.101INS、c.115T>C、c.130T>C、c.136G>T、c.159G>C、c.164G>T、c.234G>A、c.303G>A、c.333C>A和c.456C>T），其中12个与评估的性状相关。9个SNP与生理性状相关，8个与身体性状相关。选择c.136G>T作为一个新的、次要等位基因频率高且具有关联性的SNP进行基因表达研究。TT基因型个体体型匀称、生理易感性高且mRNA表达高。另一方面，GG基因型与体型较大、生理反应较低和mRNA表达低显著相关。较高的表达可能表明参与减轻应激的有害影响。结果，动物出现负能量平衡，体型减小。
单核苷酸多态性c.136G>T是巴厘牛耐热性状的候选生物标志物。
