Wan Qian, Peng Hao, Liu Feng, Liu Xiaoyi, Cheng Chuanli, Tie Changjun, Deng Jie, Lyu Jianxun, Jia Yizhen, Wang Yi, Zheng Hairong, Liang Dong, Liu Xin, Zou Chao
Paul C. Lauterbur Research Center for Biomedical Imaging, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases, Beijing International Cooperation Base for Science and Technology on NAFLD Diagnosis, Beijing, China.
Quant Imaging Med Surg. 2024 Feb 1;14(2):1591-1601. doi: 10.21037/qims-23-482. Epub 2024 Jan 2.
Gadolinium ethoxybenzyl-diethylenetriaminepentaacetic acid (Gd-EOB-DTPA) has shown potential in reflecting the hepatic function alterations in nonalcoholic steatohepatitis (NASH). The purpose of this study was to evaluate whether Gd-EOB-DTPA combined with water-specific T1 (wT1) mapping can be used to detect liver inflammation in the early-stage of NASH in rats.
In this study, 54 rats with methionine- and choline-deficient (MCD) diet-induced NASH and 10 normal control rats were examined. A multiecho variable flip angle gradient echo (VFA-GRE) sequence was performed and repeated 40 times after the injection of Gd-EOB-DTPA. The wT1 of the liver and the reduction rate of wT1 (rrT1) were calculated. All rats were histologically evaluated and grouped according to the NASH Clinical Research Network scoring system. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of Gd-EOB-DTPA transport genes. Analysis of variance and least significant difference tests were used for multiple comparisons of quantitative results between all groups. Multiple regression analysis was applied to identify variables associated with precontrast wT1 (wT1), and receiver operating characteristic (ROC) analysis was performed to assess the diagnostic performance.
The rats were grouped according to inflammatory stage (G0 =4, G1 =15, G2 =12, G3 =23) and fibrosis stage (F0 =26, F1 =19, F2 =9). After the infusion of Gd-EOB-DTPA, the rrT1 showed significant differences between the control and NASH groups (P<0.05) but no difference between the different inflammation and fibrosis groups at any time points. The areas under curve (AUCs) of rrT1 at 10, 20, and 30 minutes were only 0.53, 0.58, and 0.61, respectively, for differentiating between low inflammation grade (G0 + G1) and high inflammation grade (G2 + G3). The MRI findings were verified by qRT-PCR examination, in which the Gd-EOB-DTPA transporter expressions showed no significant differences between any inflammation groups.
The wT1 mapping quantitative method combined with Gd-EOB-DTPA was not capable of discerning the inflammation grade in a rat model of early-stage NASH.
钆乙氧基苄基 - 二乙烯三胺五乙酸（Gd - EOB - DTPA）已显示出在反映非酒精性脂肪性肝炎（NASH）肝功能改变方面的潜力。本研究的目的是评估Gd - EOB - DTPA联合水特异性T1（wT1）映射是否可用于检测大鼠NASH早期的肝脏炎症。
本研究中，对54只采用蛋氨酸和胆碱缺乏（MCD）饮食诱导的NASH大鼠和10只正常对照大鼠进行了检查。在注射Gd - EOB - DTPA后，采用多回波可变翻转角梯度回波（VFA - GRE）序列并重复40次。计算肝脏的wT1和wT1降低率（rrT1）。所有大鼠均根据NASH临床研究网络评分系统进行组织学评估和分组。采用定量实时聚合酶链反应（qRT - PCR）检测Gd - EOB - DTPA转运基因的表达。方差分析和最小显著差异检验用于所有组间定量结果的多重比较。应用多元回归分析确定与对比前wT1（wT1）相关的变量，并进行受试者操作特征（ROC）分析以评估诊断性能。
大鼠根据炎症阶段（G0 = 4，G1 = 15，G2 = 12，G3 = 23）和纤维化阶段（F0 = 26，F1 = 19，F2 = 9）进行分组。输注Gd - EOB - DTPA后，rrT1在对照组和NASH组之间显示出显著差异（P < 0.05），但在任何时间点不同炎症和纤维化组之间无差异。在区分低炎症等级（G0 + G1）和高炎症等级（G2 + G3）时，rrT1在10、20和30分钟时的曲线下面积（AUC）分别仅为0.53、0.58和0.61。MRI结果通过qRT - PCR检查得到验证，其中Gd - EOB - DTPA转运蛋白表达在任何炎症组之间均无显著差异。
Gd - EOB - DTPA联合wT1映射定量方法无法辨别早期NASH大鼠模型中的炎症等级。
