Department of Chemical & Biological Engineering, Colorado State University, Fort Collins, CO, USA.
Department of Electrical Engineering, Colorado State University, Fort Collins, CO, USA.
Trends Biotechnol. 2024 Aug;42(8):1002-1016. doi: 10.1016/j.tibtech.2024.02.002. Epub 2024 Feb 27.
In a bioeconomy that relies on synthetic DNA sequences, the ability to ensure their authenticity is critical. DNA watermarks can encode identifying data in short sequences and can be combined with error correction and encryption protocols to ensure that sequences are robust to errors and securely communicated. New digital signature techniques allow for public verification that a sequence has not been modified and can contain sufficient information for synthetic DNA to be self-documenting. In translating these techniques from bacteria to more complex genetically modified organisms (GMOs), special considerations must be made to allow for public verification of these products. We argue that these approaches should be widely implemented to assert authorship, increase the traceability, and detect the unauthorized use of synthetic DNA.
在依赖合成 DNA 序列的生物经济中，确保其真实性的能力至关重要。DNA 水印可以在短序列中编码识别数据，并可以与纠错和加密协议结合使用，以确保序列能够抵御错误并安全传输。新的数字签名技术允许公众验证序列未被修改，并且可以包含足够的信息，使合成 DNA 具有自我证明能力。在将这些技术从细菌转化为更复杂的转基因生物体 (GMO) 时，必须特别考虑允许公众对这些产品进行验证。我们认为，应该广泛采用这些方法来主张作者身份、提高可追溯性，并检测未经授权使用合成 DNA 的行为。
