Drożdż Kamil, Gołda-Cępa Monika, Chytrosz-Wróbel Paulina, Kotarba Andrzej, Brzychczy-Włoch Monika
Department of Molecular Medical Microbiology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Krakow 31-121, Poland.
Faculty of Chemistry, Jagiellonian University, Krakow 31-007, Poland.
Int J Biomater. 2024 Feb 23;2024:5102603. doi: 10.1155/2024/5102603. eCollection 2024.
Polyurethanes (PUs) are versatile polymers used in medical applications due to their high flexibility and fatigue resistance. PUs are widely used for synthetic blood vessels, wound dressings, cannulas, and urinary and cardiovascular catheters. Many scientific reports indicate that surface wettability is crucial for biocompatibility and bacterial adhesion. The use of oxygen plasma to modify PUs is advantageous because of its effectiveness in introducing oxygen-containing functional groups, thereby altering surface wettability. The purpose of this study was to investigate the effect of the modification of the oxygen plasma of polyurethane on its biocompatibility with lung tissue (A549 cell line) and the adhesion of Gram-positive bacteria ( and ). The results showed that the modification of polyurethane by oxygen plasma allowed the introduction of functional groups containing oxygen (-OH and -COOH), which significantly increased its hydrophilicity (change from 105° ± 2° to 9° ± 2°) of PUs. Surface analysis by atomic force microscopy (AFM) showed changes in PU topography (change in maximum height from ∼110.3 nm to ∼32.1 nm). Moreover, biocompatibility studies on A549 cells showed that on the PU-modified surface, the cells exhibited altered morphology (increases in cell surface area and length, and thus reduced circularity) without concomitant effects on cell viability. However, serial dilution and plate count and microscopic methods confirmed that plasma modification significantly increased the adhesion of and bacteria. This study indicate the important role of surface hydrophilicity in biocompatibility and bacterial adhesion, which is important in the design of new medical biomaterials.
聚氨酯（PUs）是一种用途广泛的聚合物，因其具有高柔韧性和抗疲劳性而被应用于医学领域。聚氨酯广泛用于合成血管、伤口敷料、插管以及泌尿和心血管导管。许多科学报告表明，表面润湿性对于生物相容性和细菌黏附至关重要。使用氧等离子体改性聚氨酯具有优势，因为它能有效引入含氧官能团，从而改变表面润湿性。本研究的目的是探究聚氨酯的氧等离子体改性对其与肺组织（A549细胞系）的生物相容性以及革兰氏阳性菌（和）黏附的影响。结果表明，通过氧等离子体对聚氨酯进行改性可引入含氧官能团（-OH和-COOH），这显著提高了聚氨酯的亲水性（接触角从105°±2°变为9°±2°）。通过原子力显微镜（AFM）进行的表面分析显示聚氨酯的形貌发生了变化（最大高度从约110.3nm变为约32.1nm）。此外，对A549细胞的生物相容性研究表明，在聚氨酯改性表面上，细胞形态发生了改变（细胞表面积和长度增加，从而圆度降低），但对细胞活力没有伴随影响。然而，系列稀释和平板计数以及显微镜方法证实，等离子体改性显著增加了和细菌的黏附。本研究表明表面亲水性在生物相容性和细菌黏附中的重要作用，这在新型医用生物材料的设计中具有重要意义。
