Hario Saaya, Le Giang N T, Sugimoto Hikaru, Takahashi-Yamashiro Kei, Nishinami Suguru, Toda Hirofumi, Li Selene, Marvin Jonathan S, Kuroda Shinya, Drobizhev Mikhail, Terai Takuya, Nasu Yusuke, Campbell Robert E
Department of Chemistry, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6, Canada.
ACS Cent Sci. 2024 Jan 31;10(2):402-416. doi: 10.1021/acscentsci.3c01250. eCollection 2024 Feb 28.
l-Lactate is a monocarboxylate produced during the process of cellular glycolysis and has long generally been considered a waste product. However, studies in recent decades have provided new perspectives on the physiological roles of l-lactate as a major energy substrate and a signaling molecule. To enable further investigations of the physiological roles of l-lactate, we have developed a series of high-performance (Δ/ = 15 to 30 ), intensiometric, genetically encoded green fluorescent protein (GFP)-based intracellular l-lactate biosensors with a range of affinities. We evaluated these biosensors in cultured cells and demonstrated their application in an preparation of brain tissue. Using these biosensors, we were able to detect glycolytic oscillations, which we analyzed and mathematically modeled.
L-乳酸是细胞糖酵解过程中产生的一种单羧酸，长期以来通常被认为是一种废物。然而，近几十年来的研究为L-乳酸作为主要能量底物和信号分子的生理作用提供了新的视角。为了能够进一步研究L-乳酸的生理作用，我们开发了一系列高性能（Δ/ = 15至30）、强度测量、基于基因编码绿色荧光蛋白（GFP）的具有一系列亲和力的细胞内L-乳酸生物传感器。我们在培养细胞中评估了这些生物传感器，并证明了它们在脑组织结构制备中的应用。使用这些生物传感器，我们能够检测糖酵解振荡，并对其进行分析和数学建模。
