Tamakoshi Keigo, Meguro Kota, Takahashi Yuri, Oshimi Ryu, Iwasaki Natsuka
Department of Physical Therapy, Niigata University of Health and Welfare.
Institute for Human Movement and Medical Sciences.
Neuroreport. 2023 Apr 5;34(6):332-337. doi: 10.1097/WNR.0000000000001898. Epub 2023 Mar 14.
In this study, we compared the mechanisms of brain recovery in intracerebral hemorrhage and ischemia, focusing on synapses, glial cells, and dopamine expression, which are considered fundamental for neural recovery after stroke. Male Wistar rats were divided into intracerebral hemorrhage, ischemia, and sham surgery (SHAM) groups. The intracerebral hemorrhage group was injected with a collagenase solution, the ischemia group was injected with an endothelin-1 solution, and the SHAM group was injected with physiological saline. The motor function of these rats was evaluated using a rotarod test on days 7, 14, 21, and 28 post-surgery. On postoperative day 29, lesion volume was analyzed using Nissl staining. In addition, the protein expression levels of NeuN, GFAP, tyrosine hydroxylase, and PSD95 were analyzed in the striatum and motor cortex. There was no significant difference between the ischemia and intracerebral hemorrhage groups in terms of lesion volume in the striatum; however, the motor recovery of the intracerebral hemorrhage group occurred more rapidly than that of the ischemia group, and the intracerebral hemorrhage group exhibited higher GFAP protein expression in the motor cortex. The rapid motor recovery in intracerebral hemorrhage rats relative to that in ischemia rats may be associated with changes in astrocytes in brain regions remote from the injury site.
在本研究中，我们比较了脑出血和缺血性脑损伤后的脑恢复机制，重点关注突触、胶质细胞和多巴胺表达，这些被认为是中风后神经恢复的基础。雄性Wistar大鼠被分为脑出血组、缺血组和假手术（SHAM）组。脑出血组注射胶原酶溶液，缺血组注射内皮素-1溶液，SHAM组注射生理盐水。在术后第7、14、21和28天，使用转棒试验评估这些大鼠的运动功能。术后第29天，采用尼氏染色分析损伤体积。此外，分析纹状体和运动皮层中NeuN、GFAP、酪氨酸羟化酶和PSD95的蛋白表达水平。纹状体损伤体积在缺血组和脑出血组之间无显著差异；然而，脑出血组的运动恢复比缺血组更快，并且脑出血组在运动皮层中表现出更高的GFAP蛋白表达。相对于缺血大鼠，脑出血大鼠的快速运动恢复可能与远离损伤部位的脑区星形胶质细胞的变化有关。
