Kokabiyan Zahra, Yaghmaei Parichehreh, Jameie Seyed Behnamedin, Hajebrahimi Zahra
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.Email:
Int J Fertil Steril. 2022 Aug 21;16(3):184-191. doi: 10.22074/ijfs.2021.537724.1176.
Polycystic ovary syndrome (PCOS) is a common type of endocrinopathy in women which is accompanied by androgens elevation, insulin resistance, and metabolic dysfunction. Eugenol is a phenolic component of clove oil that has an antioxidant, anti-inflammatory, and anti-diabetic activity. The present study aimed to evaluate the therapeutic effects of eugenol on the PCOS models of rats.
In this experimental study, thirty adults female Wistar rats weighing between 180 and 200 g were used. Estradiol valerate-induced PCOS rats (4 mg/rat) were treated with eugenol (12 and 24 mg/kg) for 28 days. The effects of eugenol were studied on levels of glucose, lipid profile, liver enzymes, reproductive hormones, oxidative stress, and the expression of cyclooxygenase-2 (Cox-2) and peroxisome proliferator-activated receptor alpha (Ppar-α) genes, using biochemical analysis of blood and histopathological evaluation of ovaries.
Results: Estradiol valerate-induced PCOS resulted in the formation of cystic follicles in the ovaries, hyperinsulinemia, hyperglycemia, hyperlipidemia, hyperandrogenism, and anovulation. It altered the Cox-2 and Ppar-α gene expression and increased oxidative stress and activities of liver enzymes. Eugenol treatment improved the PCOS-associated endocrine and metabolic disorder and histopathological alterations, mostly through antioxidant, anti-diabetic, anti hyperlipidemic, and anti-androgenic properties. It showed beneficial effects on serum glucose, serum insulin, fat profile, reproductive hormones, liver activity, oxidative stress, expression of Cox-2 and Ppar-α genes, as well as restoration of normal ovulation in the PCOS animals.
Conclusion: Eugenol could represent a promising natural product to prevent PCOS or reduce its symptoms.
多囊卵巢综合征（PCOS）是女性常见的一种内分泌疾病，伴有雄激素升高、胰岛素抵抗和代谢功能障碍。丁香酚是丁香油中的一种酚类成分，具有抗氧化、抗炎和抗糖尿病活性。本研究旨在评估丁香酚对大鼠PCOS模型的治疗效果。
在本实验研究中，使用了30只体重在180至200克之间的成年雌性Wistar大鼠。用戊酸雌二醇诱导PCOS大鼠（4毫克/只），并用丁香酚（12和24毫克/千克）治疗28天。通过血液生化分析和卵巢组织病理学评估，研究丁香酚对血糖、血脂、肝酶、生殖激素、氧化应激以及环氧合酶-2（Cox-2）和过氧化物酶体增殖物激活受体α（Ppar-α）基因表达水平的影响。
戊酸雌二醇诱导的PCOS导致卵巢中形成囊性卵泡、高胰岛素血症、高血糖、高脂血症、高雄激素血症和无排卵。它改变了Cox-2和Ppar-α基因的表达，增加了氧化应激和肝酶活性。丁香酚治疗改善了与PCOS相关的内分泌和代谢紊乱以及组织病理学改变，主要是通过抗氧化、抗糖尿病、抗高脂血症和抗雄激素特性。它对血清葡萄糖、血清胰岛素、血脂、生殖激素、肝脏活性、氧化应激、Cox-2和Ppar-α基因的表达以及PCOS动物正常排卵的恢复均显示出有益作用。
丁香酚可能是一种有前景的天然产物，可预防PCOS或减轻其症状。
