Modrzewska Marta, Popowski Dominik, Błaszczyk Lidia, Stępień Łukasz, Urbaniak Monika, Bryła Marcin, Cramer Benedikt, Humpf Hans-Ulrich, Twarużek Magdalena
Department of Food Safety and Chemical Analysis, Prof. Waclaw Dabrowski Institute of Agricultural and Food Biotechnology-State Research Institute, Rakowiecka 36, 02-532, Warsaw, Poland.
Plant Microbiomics Team, Institute of Plant Genetics, Polish Academy of Sciences, 60-479, Poznan, Poland.
Sci Rep. 2024 Mar 11;14(1):5865. doi: 10.1038/s41598-024-55920-x.
The present study assessed the ability of Trichoderma to combat F. sporotrichioides, focusing on their antagonistic properties. Tests showed that Trichoderma effectively inhibited F. sporotrichioides mycelial growth, particularly with T. atroviride strains. In co-cultures on rice grains, Trichoderma almost completely reduced the biosynthesis of T-2 and HT-2 toxins by Fusarium. T-2 toxin-α-glucoside (T-2-3α-G), HT-2 toxin-α-glucoside (HT-2-3α-G), and HT-2 toxin-β-glucoside (HT-2-3β-G) were observed in the common culture medium, while these substances were not present in the control medium. The study also revealed unique metabolites and varying metabolomic profiles in joint cultures of Trichoderma and Fusarium, suggesting complex interactions. This research offers insights into the processes of biocontrol by Trichoderma, highlighting its potential as a sustainable solution for managing cereal plant pathogens and ensuring food safety.
本研究评估了木霉菌对抗拟枝孢镰刀菌的能力，重点关注其拮抗特性。试验表明，木霉菌能有效抑制拟枝孢镰刀菌的菌丝生长，特别是绿色木霉菌株。在稻谷共培养中，木霉菌几乎完全抑制了镰刀菌的T-2和HT-2毒素的生物合成。在共同培养基中观察到了T-2毒素-α-葡萄糖苷（T-2-3α-G）、HT-2毒素-α-葡萄糖苷（HT-2-3α-G）和HT-2毒素-β-葡萄糖苷（HT-2-3β-G），而对照培养基中不存在这些物质。该研究还揭示了木霉菌和镰刀菌联合培养中的独特代谢产物和不同的代谢组学特征，表明存在复杂的相互作用。本研究为木霉菌的生物防治过程提供了见解，突出了其作为管理谷物植物病原体和确保食品安全的可持续解决方案的潜力。
