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环状RNA CircFAM188B编码一种调节鸡骨骼肌卫星细胞增殖和分化的蛋白质。

Circular RNA CircFAM188B Encodes a Protein That Regulates Proliferation and Differentiation of Chicken Skeletal Muscle Satellite Cells.

作者信息

Yin Huadong, Shen Xiaoxu, Zhao Jing, Cao Xinao, He Haorong, Han Shunshun, Chen Yuqi, Cui Can, Wei Yuanhang, Wang Yan, Li Diyan, Zhu Qing

机构信息

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.

出版信息

Front Cell Dev Biol. 2020 Nov 6;8:522588. doi: 10.3389/fcell.2020.522588. eCollection 2020.

DOI:10.3389/fcell.2020.522588
PMID:33240871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7677141/
Abstract

Circular RNAs (circRNAs) are recognized as functional non-coding transcripts; however, emerging evidence has revealed that some synthetic circRNAs generate functional peptides or proteins. Additionally, the diverse biological functions of circRNAs include acting as miRNA-binding sponges, RNA-binding protein regulators, and protein translation templates. Previously, we found that circular RNA circFAM188B is a stable circular RNA and differentially expressed between broiler chickens and layers during embryonic skeletal muscle development. In this study, we found that circFAM188B exhibited a unique pattern of sharply decreased expression from embryonic day 10 (E10) to Day 35 (D35) after hatching. Our experimental results showed that circFAM188B promotes the proliferation, but inhibits the differentiation of chicken skeletal muscle satellite cells (SMSCs). Bioinformatic analysis revealed circFAM188B contain an opening reading frame (ORF) which translate into circFAM188B-103aa, internal ribosome entry site (IRES) analysis further confirmed the coding potential of circFAM188B. In addition, western blot assay detected a flag tagged circFAM188B-103aa, and several peptides of circFAM188B-103aa were detected by LC-MS/MS analysis. We further verified that the role of circFAM188B-103aa in chicken myogenesis is consistent with that of its parent transcript circFAM188B, which facilitates proliferation, but represses differentiation of chicken SMSC. Taken together, these results suggested that a novel protein circFAM188B-103aa encoded by circFAM188B that promotes the proliferation but inhibits the differentiation of chicken SMSCs.

摘要

环状RNA（circRNAs）被认为是功能性非编码转录本；然而，新出现的证据表明，一些人工合成的circRNAs能产生功能性肽或蛋白质。此外，circRNAs的多种生物学功能包括充当miRNA结合海绵、RNA结合蛋白调节剂和蛋白质翻译模板。此前，我们发现环状RNA circFAM188B是一种稳定的环状RNA，在胚胎骨骼肌发育过程中，肉鸡和蛋鸡之间存在差异表达。在本研究中，我们发现circFAM188B在孵化后从胚胎第10天（E10）到第35天（D35）呈现出独特的急剧下降的表达模式。我们的实验结果表明，circFAM188B促进鸡骨骼肌卫星细胞（SMSC）的增殖，但抑制其分化。生物信息学分析显示，circFAM188B包含一个开放阅读框（ORF），可翻译成circFAM188B-103aa，内部核糖体进入位点（IRES）分析进一步证实了circFAM188B的编码潜力。此外，蛋白质免疫印迹分析检测到一个带有标签的circFAM188B-103aa，液相色谱-串联质谱分析检测到circFAM188B-103aa的几种肽段。我们进一步证实，circFAM188B-103aa在鸡肌生成中的作用与其亲本转录本circFAM188B一致，即促进鸡SMSC的增殖，但抑制其分化。综上所述，这些结果表明，circFAM188B编码的一种新型蛋白质circFAM188B-103aa促进鸡SMSC的增殖，但抑制其分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/985c6ca85658/fcell-08-522588-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/b4680ad2b545/fcell-08-522588-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/cf3e2aaf76c0/fcell-08-522588-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/85c95e7504e8/fcell-08-522588-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/51884492d2c5/fcell-08-522588-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/2c2c60c28c86/fcell-08-522588-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/07d46f932f8a/fcell-08-522588-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/b33a1c02dd7c/fcell-08-522588-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/e0564020bb46/fcell-08-522588-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/985c6ca85658/fcell-08-522588-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/b4680ad2b545/fcell-08-522588-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/cf3e2aaf76c0/fcell-08-522588-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/85c95e7504e8/fcell-08-522588-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/51884492d2c5/fcell-08-522588-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/2c2c60c28c86/fcell-08-522588-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/07d46f932f8a/fcell-08-522588-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/b33a1c02dd7c/fcell-08-522588-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/e0564020bb46/fcell-08-522588-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4189/7677141/985c6ca85658/fcell-08-522588-g009.jpg

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