Park Jung-Hyang, Kwag Eunbin, Jeong Mi-Kyung, Park So-Jung, Lee Sanghun, Yoo Hwa-Seung
Dunsan Korean Medicine Hospital of Daejeon University, Daejeon, Republic of Korea.
Memorial Sloan Kettering Cancer Center, New York, USA.
Integr Cancer Ther. 2024 Jan-Dec;23:15347354241233544. doi: 10.1177/15347354241233544.
In the era of precision medicine, individual temperature sensitivity has been highlighted. This trait has traditionally been used for cold-heat pattern identification to understand the inherent physical characteristics, which are influenced by genetic factors, of an individual. However, genome-wide association studies (GWASs) on this trait are limited.
Using genotype data from 90 patients with advanced non-small cell lung cancer (NSCLC) and epidermal growth factor receptor mutations, we performed a GWAS to assess the association between single nucleotide polymorphisms (SNPs) and temperature sensitivity, such as cold and heat scores. The score of each participant was evaluated using self-administered questionnaires on common symptoms and a 15-item symptom-based cold-heat pattern identification questionnaire.
The GWAS was adjusted for confounding factors, including age and sex, and significant associations were identified for cold and heat scores: SNP rs145814326, located on the intron of at chromosome 4p16.1, had a -value of 1.86 × 10; and SNP rs79297667, located upstream from at chromosome 9q22.2, had a -value of 8.97 × 10. We also found that the genetic variant regulates the expression level of in the main tissues, including the lungs and white blood cells, in NSCLC.
was found to be significantly associated with temperature sensitivity in patients with NSCLC, suggesting an increased expression of in patients with higher heat scores. The potential role of temperature sensitivity as a prognostic or predictive marker of immune response in NSCLC should be further studied.
在精准医学时代，个体温度敏感性受到了关注。传统上，这一特征被用于寒热证型辨识，以了解受遗传因素影响的个体内在生理特征。然而，针对这一特征的全基因组关联研究（GWAS）较为有限。
利用90例晚期非小细胞肺癌（NSCLC）及表皮生长因子受体突变患者的基因型数据，我们进行了一项GWAS，以评估单核苷酸多态性（SNP）与温度敏感性（如寒热评分）之间的关联。通过自我填写的常见症状问卷和一份基于15项症状的寒热证型辨识问卷对每位参与者的评分进行评估。
对年龄和性别等混杂因素进行了GWAS校正，确定了与寒热评分存在显著关联：位于4号染色体p16.1上内含子区域的SNP rs145814326，其P值为1.86×10；位于9号染色体q22.2上上游区域的SNP rs79297667，其P值为8.97×10。我们还发现该基因变异在NSCLC的主要组织（包括肺和白细胞）中调节了某基因的表达水平。
发现某基因与NSCLC患者的温度敏感性显著相关，提示热评分较高的患者中该基因表达增加。温度敏感性作为NSCLC免疫反应的预后或预测标志物的潜在作用应进一步研究。
