Changes in Tight Junction Protein Expression Levels but Not Distribution in Commercial White and Brown Laying Hens Supplemented with or Seaweed.

It is proposed that prebiotic diet supplements improve intestinal function, in part by improving the barrier function of the intestinal epithelium with an associated increase in the expression of tight junction proteins, including occludin and zonula occludens-1 (ZO-1). We examined the expression of these proteins in two strains of laying hens (Lohman LSL-lite (White) and Lohman Brown-lite (Brown)) who were supplemented or not with 3% or 0.5% seaweeds from 31 to 72 weeks of age. Occludin was localized to the lateral surfaces and across the intestinal epithelium in all animals. Reactivity for ZO-1 was concentrated at the apicolateral epithelial cell membrane border. Mood's median test indicated that White hens may express more occludin in villus epithelium (median intensity 3.5 vs. 2.5 in Brown hens, = 0.06) but less ZO-1 in the deep cryptal epithelium (median intensity 1.5 vs. 2.5 in Brown hens, = 0.06). Western blotting also showed higher levels of occludin in White than Brown hens ( < 0.05). A decrease in ZO-1 Western blot expression was associated with supplementation in comparison to controls ( < 0.05), but not with supplementation ( > 0.05). In conclusion, genetic strain and dietary seaweed supplements affect tight junction regulatory protein expression levels but do not impact the anatomical distribution, as seen in cryosections.