Faculty of Agriculture Science, Food Industry and Environmental Protection, "Lucian Blaga" University of Sibiu, 550012 Sibiu, Romania.
Faculty of Medicine, "Lucian Blaga" University of Sibiu, 550012 Sibiu, Romania.
Molecules. 2022 Aug 5;27(15):4986. doi: 10.3390/molecules27154986.
The aim of this study was to determine the variability of several chemical compounds and the antioxidant and antimicrobial activities of eight types of berries harvested from two different geographical regions in the same year. The analyses were performed on bilberry, black currant, gooseberry, red currant, raspberry, sea buckthorn, strawberry and sour cherry, which were handpicked during the summer of 2019, in the same periods when they are typically harvested for consumer purposes. Total anthocyanins content (TAC), total flavonoids content (TFC), total polyphenolic compounds (TPC), determination of the Ferric-Reducing Antioxidant Power (FRAP), determination of the DPPH free radical scavenging assay (RSA), determination of nine phenolic compounds by HPLC-UV assay and antimicrobial activity were determined for undiluted hydroalcoholic extracts of all the studied berries. The results showed that the berries from Romania were richer in antioxidant compounds than the berries from Russia. The TPC content varied between 4.13-22.2 mg GAE/g d.w., TFC between 3.33-8.87 mg QE/g d.w. and TAC between 0.13-3.94 mg/g d.w. The highest variability was determined for TPC. Regarding the antioxidant activity assessed by FRAP assay, values were between 6.02-57.23 µmols TE/g d.w. and values for the RSA method between 18.44-83.81%. From the eight types of berries analyzed, bilberries and raspberries had the highest antioxidant activity considering both regions and both determination methods. Not only the type, but also the environmental and cultivation conditions in which the berries grow, can lead to variations in their chemical composition. The extracted polyphenolic compounds from the studied berries showed antibacterial properties on pathogens, such as , and . The inhibitory action on and fungi and was absent to very low. The antimicrobial activity of the hydroalcoholic extracts was dependent on the provenance of the berries, too.
本研究旨在确定当年在两个不同地理区域收获的八种浆果中几种化合物的变异性以及抗氧化和抗菌活性。分析是在夏季 2019 年手工采摘的越橘、黑加仑、醋栗、红加仑、覆盆子、沙棘、草莓和酸樱桃上进行的，这与消费者通常收获这些浆果的时间相同。总花青素含量 (TAC)、总类黄酮含量 (TFC)、总多酚含量 (TPC)、铁还原抗氧化能力 (FRAP) 的测定、DPPH 自由基清除 assay (RSA) 的测定、通过 HPLC-UV 测定法测定的九种酚类化合物和抗菌活性均为所有研究浆果的未稀释水醇提取物。结果表明，罗马尼亚的浆果比俄罗斯的浆果含有更丰富的抗氧化化合物。TPC 含量在 4.13-22.2mgGAE/gdw 之间，TFC 在 3.33-8.87mgQE/gdw 之间，TAC 在 0.13-3.94mg/gdw 之间。TPC 的变化最大。关于 FRAP 测定法评估的抗氧化活性，值在 6.02-57.23µmolsTE/gdw 之间，RSA 法的值在 18.44-83.81%之间。在所分析的八种浆果中，考虑到两个地区和两种测定方法，越橘和覆盆子的抗氧化活性最高。不仅是浆果的类型，还有浆果生长的环境和种植条件，都会导致其化学成分发生变化。从研究浆果中提取的多酚化合物对病原体具有抗菌特性，如 、 和 。对 和真菌 和 的抑制作用不存在或非常低。水醇提取物的抗菌活性也取决于浆果的来源。
