Alecu Andreia, Albu Camelia, Badea Georgiana-Ileana, Alionte Aurelia, Enache Alin-Alexandru, Radu Gabriel-Lucian, Litescu Simona-Carmen
Centre of Bioanalysis, National Institute of Research and Development for Biological Sciences, 060031 Bucharest, Romania.
S.C. Apel Laser S.R.L., 077135 Mogosoaia, Romania.
Int J Mol Sci. 2025 Jan 24;26(3):992. doi: 10.3390/ijms26030992.
The extraction of bio-compounds from medicinal plants provides opportunities for using the plant extract for health benefits. L. is considered a "natural superfood", and the valorization of its active compounds requires an extraction technique that ensures a suitable extraction yield while preserving the compounds' activity. In our study, infrared laser irradiation (IRLIR) technology was used for the first time in the bioactive compound's extraction from L. Different solvents (water-ethanol, hexane-ethanol) and different extraction times were tested to obtain a high extraction yield. Chromatographic and spectrophotometry methods were used to monitor the profile of bioactive compounds and the antioxidant activity of the extracts. The results obtained for IRLIR were compared with those obtained by accelerated solvent extraction (ASE), an advanced extraction method. The IRLIR technology proved to be a more reliable analytical tool for the extraction of (+)-catechin, gallic acid, and lutein. In addition, a richer extract formula was obtained by IRLIR extraction with respect to ASE, with the IRLIR process ensuring a short extraction time, low volume of the extraction solvent, low energy consumption, and a less expensive device.
从药用植物中提取生物化合物为将植物提取物用于健康益处提供了机会。L.被认为是一种“天然超级食物”,其活性化合物的增值需要一种提取技术,该技术能确保合适的提取率,同时保留化合物的活性。在我们的研究中,红外激光辐照(IRLIR)技术首次用于从L.中提取生物活性化合物。测试了不同的溶剂(水 - 乙醇、己烷 - 乙醇)和不同的提取时间,以获得高提取率。采用色谱法和分光光度法监测生物活性化合物的概况以及提取物的抗氧化活性。将IRLIR获得的结果与通过加速溶剂萃取(ASE)这一先进提取方法获得的结果进行比较。事实证明,IRLIR技术是提取(+) - 儿茶素、没食子酸和叶黄素更可靠的分析工具。此外,相对于ASE,通过IRLIR提取获得了更丰富的提取物配方,IRLIR工艺确保了较短的提取时间、较低的提取溶剂体积、较低的能耗以及较便宜的设备。