Sherstyukova Ekaterina, Sergunova Viktoria, Kandrashina Snezhanna, Chernysh Aleksandr, Inozemtsev Vladimir, Lomakina Galina, Kozlova Elena
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia.
Faculty of Chemistry, Lomonosov Moscow State University, Lenin Hills 1/3, 119991 Moscow, Russia.
Cells. 2024 Feb 27;13(5):411. doi: 10.3390/cells13050411.
Xenon, an inert gas commonly used in medicine, has been considered as a potential option for prolonged preservation of donor packed red blood cells (pRBCs) under hypoxic conditions. This study aimed to investigate how xenon affects erythrocyte parameters under prolonged storage. In vitro model experiments were performed using two methods to create hypoxic conditions. In the first method, xenon was introduced into bags of pRBCs which were then stored for 42 days, while in the second method, xenon was added to samples in glass tubes. The results of our experiment showed that the presence of xenon resulted in notable alterations in erythrocyte morphology, similar to those observed under standard storage conditions. For pRBC bags, hemolysis during storage with xenon exceeded the acceptable limit by a factor of six, whereas the closed-glass-tube experiment showed minimal hemolysis in samples exposed to xenon. Notably, the production of deoxyhemoglobin was specific to xenon exposure in both cell suspension and hemolysate. However, this study did not provide evidence for the purported protective properties of xenon.
氙是一种常用于医学的惰性气体，一直被视为在缺氧条件下长期保存供体浓缩红细胞（pRBCs）的一种潜在选择。本研究旨在探讨氙在长期储存过程中如何影响红细胞参数。使用两种方法创造缺氧条件进行体外模型实验。第一种方法是将氙引入pRBCs袋中，然后储存42天，而第二种方法是将氙添加到玻璃管中的样本中。我们的实验结果表明，氙的存在导致红细胞形态发生显著改变，类似于在标准储存条件下观察到的情况。对于pRBC袋，在氙存在下储存期间的溶血超过可接受限度六倍，而封闭玻璃管实验显示暴露于氙的样本中溶血极少。值得注意的是，脱氧血红蛋白的产生在细胞悬液和溶血产物中均特定于氙暴露。然而，本研究并未为氙所谓的保护特性提供证据。
