Hovind-Hougen K, Nielsen H A, Birch-Andersen A
Acta Pathol Microbiol Scand C. 1979 Jun;87C(3):217-22.
The ultrastructure of cells of T. pallidum Nichols subjected to the TPI test was studied in negatively stained specimens. Cells incubated in basal medium to which was added either human syphilis serum or heated guineapig serum (GPS) showed a normal morphology. This was also the cause for cells incubated with basal medium to which was added either human syphilis serum and heated GPS or normal human serum and unheated GPS. By dark-field microscopy cells obtained from these different incubation mixtures were found to be motile. In contrast, cells incubated in basal medium to which was added human syphilis serum and unheated GPS were all immobilized, and in the electron microscope they presented a morphology strikingly different from that of normal cells. The immunoimmobilized cells were swollen and their surface was completely covered with a layer of fuzzy material. The nature of this material and its possible role in rendering the treponemes immobile is discussed.
在负染标本中研究了接受梅毒螺旋体活动抑制试验（TPI）的梅毒螺旋体Nichols株细胞的超微结构。在添加了人梅毒血清或热灭活豚鼠血清（GPS）的基础培养基中孵育的细胞呈现正常形态。这也是在添加了人梅毒血清和热灭活GPS或正常人血清和未加热GPS的基础培养基中孵育的细胞的情况。通过暗视野显微镜观察发现，从这些不同孵育混合物中获得的细胞具有运动性。相比之下，在添加了人梅毒血清和未加热GPS的基础培养基中孵育的细胞全部被固定，并且在电子显微镜下它们呈现出与正常细胞截然不同的形态。免疫固定的细胞肿胀，其表面完全覆盖着一层模糊物质。讨论了这种物质的性质及其使梅毒螺旋体失去运动能力的可能作用。
