Gao Xinwei, Cai Songtao, Wang Luwei, Guo Yong, Liu Liwei, Weng Xiaoyu, Huang Kun, Yan Wei, Qu Junle
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
School of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, China.
Biomed Opt Express. 2024 Feb 13;15(3):1595-1604. doi: 10.1364/BOE.507770. eCollection 2024 Mar 1.
Stimulated emission depletion (STED) microscopy holds tremendous potential and practical implications in the field of biomedicine. However, the weak anti-bleaching performance remains a major challenge limiting the application of STED fluorescent probes. Meanwhile, the main excitation wavelengths of most reported STED fluorescent probes were below 500 nm or above 600 nm, and few of them were between 500-600 nm. Herein, we developed a new tetraphenyl ethylene-functionalized rhodamine dye (TPERh) for mitochondrial dynamic cristae imaging that was rhodamine-based with an excitation wavelength of 560 nm. The TPERh probe exhibits excellent anti-bleaching properties and low saturating stimulated radiation power in mitochondrial STED super-resolution imaging. Given these outstanding properties, the TPERh probe was used to measure mitochondrial deformation, which has positive implications for the study of mitochondria-related diseases.
受激发射损耗（STED）显微镜在生物医学领域具有巨大的潜力和实际意义。然而，抗光漂白性能较弱仍然是限制STED荧光探针应用的主要挑战。同时，大多数已报道的STED荧光探针的主要激发波长低于500nm或高于600nm，其中很少有在500 - 600nm之间的。在此，我们开发了一种用于线粒体动态嵴成像的新型四苯基乙烯功能化罗丹明染料（TPERh），它基于罗丹明，激发波长为560nm。在 mitochondrial STED 超分辨率成像中，TPERh 探针表现出优异的抗光漂白性能和低饱和受激发射功率。鉴于这些优异性能，TPERh 探针被用于测量线粒体变形，这对线粒体相关疾病的研究具有积极意义。
