Yang Peng-Fei, Wu Wei, Han Hang-Hang, Wang Qing-Fu, Li Lei, Wang Ze-Ning, Mao Duo-Bin, Chen Zhi-Fei
College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, China.
Technology Center, China Tobacco Shaanxi Industrial Co., Ltd, Xian, China.
Nat Prod Res. 2025 Jul;39(14):4050-4054. doi: 10.1080/14786419.2024.2331042. Epub 2024 Mar 19.
Two new megastigmane glycosides, (6 ,7,9)-3-oxo--ionyl-9-O--L-rhamnopyranosyl-(1''→4')--D-glucopyranoside () and (6 ,7,9)-3-oxo--ionyl-9-O--D-glucopyranosyl-(1''→6')--D-glucopyranoside (), together with six known analogues (-) were isolated from the leaves of . The structures of all metabolites were determined by comprehensive analysis of NMR and MS spectroscopic data as well as by comparison with those of previously reported. The anti-inflammatory activity of all isolates was evaluated using a lipopolysaccharide (LPS)-induced RAW264.7 cell inflammatory model, and the compounds , , , and exhibited inhibition of LPS-induced NO production in RAW264.7 macrophage cells with IC values of 42.3-61.7 μM (positive control, dexamethasone, IC = 21.3 ± 1.2 μM).
从[植物名称]的叶子中分离出两种新的巨大戟烷糖苷，即(6 ,7,9)-3-氧代-α-紫罗兰醇-9-O-α-L-鼠李吡喃糖基-(1''→4')-β-D-吡喃葡萄糖苷（）和(6 ,7,9)-3-氧代-α-紫罗兰醇-9-O-β-D-吡喃葡萄糖基-(1''→6')-β-D-吡喃葡萄糖苷（），以及六种已知类似物（-）。通过对核磁共振（NMR）和质谱（MS）光谱数据的综合分析以及与先前报道的结构进行比较，确定了所有代谢产物的结构。使用脂多糖（LPS）诱导的RAW264.7细胞炎症模型评估了所有分离物的抗炎活性，化合物、、、和在RAW264.7巨噬细胞中对LPS诱导的NO产生具有抑制作用，IC值为42.3 - 61.7μM（阳性对照，地塞米松，IC = 21.3 ± 1.2μM）。
