Berrones Reyes Jessica, Sherin Peter S, Sarkar Amrita, Kuimova Marina K, Vilar Ramon
Department of Chemistry Imperial College London White City Campus 82 Wood Lane London W12 0BZ UK.
Angew Chem Weinheim Bergstr Ger. 2023 Oct 16;135(42):e202310402. doi: 10.1002/ange.202310402. Epub 2023 Sep 8.
G-quadruplex DNA is a non-canonical structure that forms in guanine-rich regions of the genome. There is increasing evidence showing that G-quadruplexes have important biological functions, and therefore molecular tools to visualise these structures are important. Herein we report on a series of new cyclometallated platinum(II) complexes which, upon binding to G-quadruplex DNA, display an increase in their phosphorescence, acting as switch-on probes. More importantly, upon binding to G-quadruplexes they display a selective and distinct lengthening of their emission lifetime. We show that this effect can be used to selectively visualise these structures in cells using Phosphorescence Lifetime Imaging Microscopy (PLIM).
G-四链体DNA是一种在基因组富含鸟嘌呤区域形成的非经典结构。越来越多的证据表明，G-四链体具有重要的生物学功能，因此用于可视化这些结构的分子工具很重要。在此，我们报道了一系列新型环金属化铂（II）配合物，它们与G-四链体DNA结合后，磷光增强，可作为开启型探针。更重要的是，与G-四链体结合后，它们的发射寿命会有选择性地显著延长。我们表明，这种效应可用于通过磷光寿命成像显微镜（PLIM）在细胞中选择性地可视化这些结构。
