Peanut Research Institute, College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China.
College of Agriculture and Horticulture, Liaoning Agriculture Ovcational and Technical College, Yingkou 115009, China.
Genomics. 2024 May;116(3):110835. doi: 10.1016/j.ygeno.2024.110835. Epub 2024 Mar 22.
Pod length (PL) is one of the major traits determining pod size and yield of peanut. Discovering the quantitative trait loci (QTL) and identifying candidate genes associated with PL are essential for breeding high-yield peanut. In this study, quantitative trait loci sequencing (QTL-seq) was performed using the F population constructed by a short-pod variety Tifrunner (Tif) and a long-pod line Lps, and a 0.77 Mb genomic region on chromosome 07 was identified as the candidate region for PL. Then, the candidate region was narrowed to a 265.93 kb region by traditional QTL approach. RNA-seq analysis showed that there were four differentially expressed genes (DEGs) in the candidate region, among which Arahy.PF2L6F (AhCDC48) and Arahy.P4LK2T (AhTAA1) were speculated to be PL-related candidate genes. These results were informative for the elucidation of the underlying regulatory mechanism in peanut pod length and would facilitate further identification of valuable target genes.
荚长（PL）是决定花生荚果大小和产量的主要性状之一。发现与 PL 相关的数量性状位点（QTL）和候选基因对于培育高产花生至关重要。在这项研究中，使用由短荚品种 Tifrunner（Tif）和长荚系 Lps 构建的 F 群体进行了数量性状位点测序（QTL-seq），并鉴定出 07 号染色体上的 0.77 Mb 基因组区域为 PL 的候选区域。然后，通过传统的 QTL 方法将候选区域缩小到 265.93 kb 区域。RNA-seq 分析表明，候选区域有四个差异表达基因（DEGs），其中 Arahy.PF2L6F（AhCDC48）和 Arahy.P4LK2T（AhTAA1）被推测为与 PL 相关的候选基因。这些结果为阐明花生荚长的潜在调控机制提供了信息，并将有助于进一步鉴定有价值的目标基因。
